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生物来源
Porcine heart
质量水平
形式
ammonium sulfate suspension
比活
≥200 units/mg protein
UniProt登记号
异质活性
glutamic-oxalacetic transaminase ≤0.1%
malic dehydrogenase, glutamic-pyruvic transaminase and pyruvate kinase ≤0.06%
储存温度
2-8°C
基因信息
pig ... LDHA(407245)
生化/生理作用
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
单位定义
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
外形
Suspension in ammonium sulfate and 0.1 M potassium phosphate, pH 7.0
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Clinical Biochemistry and Hematology
Washington IM and Hoosier GV
Journal of Separation Science, 57-116 (2012)
Biochemistry, Lactate Dehydrogenase
Farhana A and Lappin SL
StatPearls [Internet] (2023)
Joe T Sharick et al.
Scientific reports, 8(1), 5456-5456 (2018-04-05)
While NAD(P)H fluorescence lifetime imaging (FLIM) can detect changes in flux through the TCA cycle and electron transport chain (ETC), it remains unclear whether NAD(P)H FLIM is sensitive to other potential fates of glucose. Glucose carbon can be diverted from
J A Read et al.
Proteins, 43(2), 175-185 (2001-03-29)
Lactate dehydrogenase (LDH) interconverts pyruvate and lactate with concomitant interconversion of NADH and NAD(+). Although crystal structures of a variety of LDH have previously been described, a notable absence has been any of the three known human forms of this
Minh Vu Chuong Nguyen et al.
Biochemical pharmacology, 85(11), 1644-1654 (2013-04-16)
NADPH oxidase Nox4 is expressed in a wide range of tissues and plays a role in cellular signaling by providing reactive oxygen species (ROS) as intracellular messengers. Nox4 oxidase activity is thought to be constitutive and regulated at the transcriptional
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
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