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生物来源
Pseudomonas aeruginosa (10)
质量水平
形式
lyophilized powder
纯化方式
trichloroacetic acid extraction
杂质
1-10% Protein (Lowry)
颜色
white to yellow
溶解性
water: 4.90-5.10 mg/mL, hazy to turbid, colorless to light yellow
运输
ambient
储存温度
2-8°C
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一般描述
本品为绿脓杆菌血清型10提取的 TCA。来源菌株为 ATCC 27316。
应用
脂多糖 (LPS) 是革兰氏阴性菌细胞壁的特征性成分。LPS 及其脂质 A 部分通过 Toll 样受体 4 (TLR4) 刺激先天免疫系统的细胞，TLR4 是 Toll 样受体蛋白家族的成员之一，可识别常见的病原体相关分子模式 (PAMP)。
生化/生理作用
脂多糖（LPS）位于膜的外层，并且在非包封的菌株中，其暴露于细胞表面。它们有助于外膜保持完整，并可保护细胞免受胆汁盐和亲脂性抗生素的影响。
制备说明
本品可溶于水（5 mg/mL）或细胞培养基（1 mg/mL），产生淡黄色浑浊溶液。涡旋并加热至 70-80 o C 后，在盐水中获得了更浓缩但仍浑浊的溶液（20 mg/mL）。脂多糖是在每种溶剂中形成胶束的分子。在水和磷酸盐缓冲盐水中观察到浑浊溶液。有机溶剂不能提供更清晰的溶液。甲醇产生浑浊、带漂浮物的混悬液，而水产生均匀的浑浊溶液。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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商品
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
相关内容
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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