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Merck
CN

L6274

层粘连蛋白 来源于人类胎盘

liquid, BioReagent, suitable for cell culture

别名:

Cell culture grade laminin, Human Laminin

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关于此项目

化学文摘社编号:
114956-81-9
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00081739

主要文件

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产品名称

层粘连蛋白 来源于人类胎盘, liquid, BioReagent, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

biological source

human placenta

product line

BioReagent

form

liquid

concentration

0.5 mg/mL in Tris-buffered saline

surface coverage

1‑2 μg/cm2

UniProt accession no.

P07942

shipped in

dry ice

storage temp.

−70°C

Quality Level

300

Gene Information

human ... LAMB1(3912)

Application

层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。 建议将其按1-2 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。

Biochem/physiol Actions

层粘连蛋白是动物组织中结构性支架的组成部分。 它们通过内功素和基底膜聚糖与IV型胶原结合，并通过整合素受体、dystroglycan糖蛋白复合物以及Lutheran血型糖蛋白与细胞膜结合。 层粘连蛋白具有胶原结合、细胞粘附、肝素结合和神经突向外生长片段的活性结构域。

Disclaimer

推荐在-70℃储存该产品，它能保持稳定2年。

Other Notes

层粘连蛋白是一种细胞外基质多结构域三聚体糖蛋白，并且是支持粘附、增殖和分化的基底膜的主要非胶原组分。 层粘连蛋白是由通过多个二硫键连接的A、B1和B2链组成。使用胃蛋白酶处理和免疫亲和层析，层连蛋白产品从人胎盘纯化获得。 SDS-PAGE在50kDa和130-160kDa得到两条主要条带。

Preparation Note

该产品是在含有150 mM NaCl的50 mM Tris-HCl中以0.5 mg/mL的浓度提供。 它被0.2μm过滤。在使用前在2-8°C将该溶液缓慢解冻以避免形成凝胶。 作为一种涂层溶液，在灭菌平衡盐溶液中稀释，涂层结构表面有最小体积，在37℃培育2小时。 在细胞铺板之前用PBS洗涤3次。层粘连蛋白可储存在2-8°C达一个月。

存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000486057
0000486057
0000442161
0000442161
0000258930

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do you prepare Product L6274, Laminin from human placenta?

    To prepare a laminin solution, we recommend that the L6274 be thawed at 2-8°C to avoid the formation of a gel. This can be further diluted in a balanced salt solution. If the product has been thawed at room temperature and ends up as a gel, it cannot be reactivated for use.

  6. How long can cover slips coated with Product L6274, Laminin from human placenta, be stored?

    Laminin coatings, such as laminin coated cover slips last about one month at 2-8°C. Do not use the product if discoloration or spider web formations appear on the surface of the coated material.

  7. Is Product L6274, Laminin from human placenta, laminin 1 or 2?

    Fifteen laminin trimers have been identified. We do not determine what type of laminin this is. Typically laminin from placenta is considered laminin-2/4 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane is laminin-1.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Satu Marja Myllymäki et al.
PloS one, 6(5), e19453-e19453 (2011-05-17)
Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have
Terhi P Teräväinen et al.
PloS one, 8(8), e71485-e71485 (2013-08-27)
The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were
Pere Roca-Cusachs et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(38), 16245-16250 (2009-10-07)
A key molecular link between cells and the extracellular matrix is the binding between fibronectin and integrins alpha(5)beta(1) and alpha(v)beta(3). However, the roles of these different integrins in establishing adhesion remain unclear. We tested the adhesion strength of fibronectin-integrin-cytoskeleton linkages
Elizabeth K Davis et al.
Neural development, 3, 32-32 (2008-11-07)
Wnt proteins comprise a large class of signaling molecules that regulate a variety of developmental processes, including synapse formation. Previous studies have shown Wnts to be involved in both the induction and prevention of synapses in a number of different
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实验方案

Laminin Coating Protocol for Cell Culture

Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.

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