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生物来源
bacterial (Arthrobacter luteus)
质量水平
表单
lyophilized powder
比活
≥200 units/mg solid
技术
cell based assay: suitable
适用性
suitable for cell lysis
应用
diagnostic assay manufacturing
储存温度
−20°C
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应用
藤黄节杆菌(Arthrobacter luteus)溶壁酶可用于重悬细胞以提取高分子量DNA用于PCR（聚合酶链式反应）指纹分析。
藤黄节杆菌（Arthrobacter luteus）裂解酶已用于：
- 作为球状体形成形缓冲液成分，制备酵母核提取物
- 消化样品制剂，用酵母进行β-葡聚糖酶促测定
- 裂解细胞以便RNA分离
生化/生理作用
溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可切开糖基之间的β (1→3)和β (1→4)键。
溶壁酶是一种裂解酶，用于通过诱导部分原生质球形成，从酵母细胞中提取DNA。随后再裂解原生质球以释放DNA。酵母细胞的细胞壁可能形成荚膜或耐药孢子，很难破坏，因此溶壁酶成为消化酵母细胞壁的首选方案。溶壁酶含有β-(1→;3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。它适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
单位定义
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
其他说明
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
A protein transformation protocol for introducing yeast prion particles into yeast
Tanaka M, et al.
Methods in Enzymology, 470, 681-693 (2010)
Naturally occurring novel promoters around pyruvate branch-point for recombinant protein production in Pichia pastoris (Komagataella phaffii): pyruvate decarboxylase-and pyruvate kinase-promoters
Massahi A and Calik P
Biochemical Engineering Journal, 138, 111-120 (2018)
M Groenewald et al.
Persoonia, 21, 17-27 (2008-12-01)
The type species of the genus Debaryomyces, Debaryomyces hansenii, is a highly heterogeneous species. It has been isolated from a large diversity of natural sources including fruit, air, water, soil, but most frequently from processed food products. The species delineation
Lisbeth Rosager Poulsen et al.
The Plant cell, 20(3), 658-676 (2008-03-18)
Vesicle budding in eukaryotes depends on the activity of lipid translocases (P(4)-ATPases) that have been implicated in generating lipid asymmetry between the two leaflets of the membrane and in inducing membrane curvature. We show that Aminophospholipid ATPase3 (ALA3), a member
Alba Espargaró et al.
Microbial cell factories, 11, 89-89 (2012-06-27)
Prions were first identified as infectious proteins associated with fatal brain diseases in mammals. However, fungal prions behave as epigenetic regulators that can alter a range of cellular processes. These proteins propagate as self-perpetuating amyloid aggregates being an example of
实验方案
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。
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