一般描述
乳酸脱氢酶(LDH)是一种糖酵解酶。它存在于骨骼肌、心脏、肝脏、肾脏、大脑、肺和红细胞中。它由五种同工酶组成。LDH具有四聚体结构。
应用
使用牛心L-乳酸脱氢酶:
- 作为参考标准物,测定LDH(乳酸脱氢酶)
- 淬灭细胞中与蛋白质结合的NADH
- 测定细胞在悬浮液中释放的乳酸
用于酶法测定乳酸或丙酮酸。
生化/生理作用
乳酸脱氢酶(LDH)有助于乳酸可逆转化为丙酮酸。
还可催化其他 L-2-羟基单羧酸的氧化反应。
单位定义
在 pH 7.5,37℃ 下,一个单位每分钟将 1.0 μm 的丙酮酸盐还原成 L-乳酸盐。
外形
悬浮于 2.2M 硫酸铵中
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
实验方案
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
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