生物来源
Escherichia coli (O111:B4)
质量水平
形式
lyophilized powder
颜色
white to beige
溶解性
water: soluble
运输
ambient
储存温度
2-8°C
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一般描述
本品从 O111:B4 血清型大肠杆菌中提取，经色谱法纯化。来源菌株来自私人收藏。脂质 A 部分已通过碱水解部分脱脂。去除类脂 A 的脂肪酸部分可产生一种去毒 LPS，其内毒素级别比母体 LPS 低 10,000 倍。
应用
脂多糖（LPS）是革兰氏阴性菌细胞壁的特征组分。LPS及其脂质A部分可通过能够识别常见的病原体相关分子模式（PAMP）的Toll样受体蛋白家族成员Toll样受体4（TLR4）刺激先天性免疫系统细胞。
生化/生理作用
脂多糖（LPS）位于膜的外层，并且在非包封的菌株中，其暴露于细胞表面。它们有助于外膜的完整性，并可保护细胞免受胆汁盐和亲脂性抗生素的作用。
制备说明
色谱纯化；碱水解脱脂
该产品可溶于水（5mg / ml）或细胞培养基（1mg / ml），产生浑浊的淡黄色溶液。当涡旋并升温至70-80 oC后，在盐水中可获得更高浓度的溶液（20mg/ml），但仍然是浑浊的溶液。脂多糖是一种在各种溶剂中都形成胶束的分子。在水和磷酸盐缓冲盐水中可观察到浑浊的溶液。有机溶剂无法使溶液变澄清。甲醇会产生含有漂浮物的混浊悬浮液，而水会产生均匀的混浊溶液。
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产品编号
说明
价格
警示用语：
Danger
危险声明
危险分类
Acute Tox. 2 Oral
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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相关内容
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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