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L2630

Sigma-Aldrich

脂多糖 来源于大肠杆菌 0111:B4

purified by phenol extraction

别名:

LPS

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About This Item

EC 号:
297-473-0
MDL编号:
MFCD00164401
UNSPSC代码:
12352201
NACRES:
NA.25

生物来源

Escherichia coli (O111:B4)

质量水平

300

形式

lyophilized powder

纯化方式

phenol extraction

技术

ELISA: suitable
cell based assay: suitable
ligand binding assay: suitable

杂质

≤3.00% protein (Lowry-TCA)

颜色

off-white to faint yellow

溶解性

water: soluble

应用

cell analysis
clinical research
life science and biopharma

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

脂多糖是一种糖脂，由与脂肪酸连接的碳水化合物组成。脂多糖（LPS）是革兰氏阴性菌细胞壁的特征性成分。典型的LPS分子具有脂质A（一种基于葡糖胺的磷脂）、一个相对短的核心寡糖以及一个远端的多糖（O-抗原）。LPS有助于外膜的完整性，并可保护细胞免受胆汁盐和亲脂性抗生素的作用。它们通过TLR2和TLR4（toll样受体）对宿主细胞进行刺激。这种刺激可导致各种促炎细胞因子的生成，例如TNFα（肿瘤坏死因子）、IL-1（白细胞介素1）和IL-6。
该产品是从大肠杆菌血清型O111:B4中经酚抽提的。来源菌株来自私人保藏。该LPS血清型已用于刺激B细胞并在人肝细胞中诱导NOS（ 一氧化氮合酶）。

应用

来源于大肠杆菌 O111:B4的脂多糖(LPS)已用于：

  • 刺激巨噬细胞
  • 小鼠 LPS 预处理
  • 诱导小鼠急性肾损伤
  • 诱导小鼠急性呼吸窘迫综合征 (ARDS) ，以研究骨髓间充质干细胞 (BMSC) 来源外泌体的抗炎作用
  • 诱导重症急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 感染小鼠的高炎症状态
脂多糖（LPS）是革兰氏阴性菌细胞壁的特征组分。LPS及其脂质A部分可通过能够识别常见的病原体相关分子模式（PAMP）的Toll样受体蛋白家族成员Toll样受体4（TLR4）刺激先天性免疫系统细胞。

生化/生理作用

脂多糖 (LPS) 是外膜的组成部分，使外膜可透过疏水性分子，如使革兰氏阴性菌具有天然耐药性的抗菌物质。LPS 参与细菌-宿主相互作用，调节宿主系统反应。

制备说明

该产物可溶于水 (5 mg/ml)或细胞培养基（1 mg/ml），产生模糊的淡黄色溶液。以中更为浓缩但仍然模糊的溶液（20 mg/ml）可形成于涡旋并升温至70-80oC后。脂多糖是在每种溶剂中都形成胶束的分子。在水和磷酸盐缓冲液中都能观察到模糊的溶液。有机溶剂也不会产生更为清澈的溶液。甲醇可产生具有漂浮物的混浊悬浮液，而水则能产生均匀模糊的溶液。

其他说明

为了全面了解我们针对客户研究提供的各种脂多糖产品，建议您访问我们的碳水化合物分类页面。
如需了解生化试剂系列的更多信息，请填写此表。

相关产品

产品编号
说明
价格

象形图

Skull and crossbones
GHS06

警示用语：

Danger

危险声明

H300

危险分类

Acute Tox. 2 Oral

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How concentrated a stock solution can I make of Product L2630, Lipopolysaccarides (LPS)?

    One can try to make a 20 mg/mL solution at 70-80°C in saline, with vortexing and warming.  However, the solution will be hazy, because of the nature of LPS to form micelles in solutions like water or PBS.

  6. What is the importance of the "purified by phenol extraction" description for Product L2630, Lipopolysaccarides (LPS)?

    Different extraction methods for LPS leave different levels of potential nucleic acid and protein contaminations.  Phenol extraction can leave up to 60% RNA and <1% protein present.

  7. What is the molecular weight of Product L2630, Lipopolysaccarides (LPS)?

    We have not determined the molecular weight of this item. One reference, J. Biol. Chem., 250, 2911 (1975), states approximate values of 90,000 and 0.9-1.5 million, by both sedimentation velocity and gel filtration, for two forms of LPS that may be present.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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