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安全信息

L2524

Sigma-Aldrich

溶壁酶 来源于藤黄节杆菌

lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

别名:

细菌溶壁酶, 裂解酶

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About This Item

CAS号:
37340-57-1
MDL编号:
MFCD00131560
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

bacterial (Arthrobacter luteus)

质量水平

300

形式

lyophilized powder

比活

≥2,000 units/mg protein

组成

Protein, ≥20% biuret

技术

cell based assay: suitable

适用性

suitable for cell lysis

应用

diagnostic assay manufacturing

储存温度

−20°C

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相关类别

应用

藤黄节杆菌(Arthrobacter luteus)溶壁酶用于：
  • 制备原生质球
  • 作为消化液组分，用于孵育酵母细胞消化细胞壁
  • 酶解菌丝体沉淀以制备原生质体

藤黄节杆菌（Arthrobacter luteus ）裂解酶已用于裂解真菌细胞壁，分离DNA。

生化/生理作用

溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可断链糖基之间的β(1→3)和β(1→4)键。溶菌酶是巨噬细胞介导宿主反应的指示剂，与白细胞死亡有关，具有高周转率。多种慢性炎症、炎症性肠病、血液病和肾病中都发现血清溶菌酶浓度提高。鸡蛋白中的C型溶菌酶常用作蛋白结构和功能的研究模型。胞壁质酶主要对革兰氏阳性菌具有杀菌活性。手册所述凝胶过滤色谱法测定分子量是现有发表方法的改良版。该试剂盒中的蛋白标准品可能适合其他色谱方法，如高效液相色谱(HPLC)。但是，某些缓冲体系可能会影响白蛋白和碳酸酐酶的洗脱体积。该试剂盒中的蛋白分子量范围为29 kDa至699 kDa。
酵母细胞很难被破坏，因为其细胞壁可能形成胶囊或耐药孢子。可通过裂解酶（如溶壁酶）诱导部分原生质球形成，从酵母中提取DNA。随后再裂解原生质球以释放DNA。溶壁酶更易消化酵母菌细胞壁，并从真菌中产生原生质球进行转化。它含有β-(1→3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。溶壁酶适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。

单位定义

一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。

外形

部分纯化的冻干粉末，含有磷酸钾缓冲盐和稳定剂

其他说明

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I solubilize Product L2524, Lyticase?

    The quality control enzymatic assay protocol for Lyticase uses cold deionized water to make a solution at 500 units/mL.

  4. How can I store Product L2524, Lyticase, once reconstituted?

    When lyticase is reconstituted and stored, it may be stable for no more than 4-6 hours before serious loss of activity is observed. It is suggested that fresh solutions be prepared for use.

  5. How can Product L2524, Lyticase, be used to make yeast spheroplasts?

    The use of lyticase in combination with chitinase and glucuronidase in the preparation of spheroplasts from C. albicans has been described in the following reference:  Calvert, C.M., and Sanders, D., "Inositol Trisphosphate-dependent and -independent Ca2+ Mobilization Pathways at the Vacuolar Membrane of Candida albicans". J. Biol. Chem.  270, 7272-7280 (1995).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Daniel Zenklusen et al.
Nature structural & molecular biology, 15(12), 1263-1271 (2008-11-18)
Proper execution of transcriptional programs is a key requirement of gene expression regulation, demanding accurate control of timing and amplitude. How precisely the transcription machinery fulfills this task is not known. Using an in situ hybridization approach that detects single
A protein transformation protocol for introducing yeast prion particles into yeast
Tanaka M, et al.
Methods in Enzymology, 470, 681-693 (2010)
Bing Zhai et al.
Nature medicine, 26(1), 59-64 (2020-01-08)
The intestinal microbiota is a complex community of bacteria, archaea, viruses, protists and fungi1,2. Although the composition of bacterial constituents has been linked to immune homeostasis and infectious susceptibility3-7, the role of non-bacterial constituents and cross-kingdom microbial interactions in these
Hagen Frickmann et al.
BMC microbiology, 19(1), 75-75 (2019-04-10)
The potential of next-generation sequencing (NGS) for hypothesis-free pathogen diagnosis from (poly-)microbially contaminated, formalin-fixed, paraffin embedded tissue samples from patients with invasive fungal infections and amebiasis was investigated. Samples from patients with chromoblastomycosis (n = 3), coccidioidomycosis (n = 2), histoplasmosis (n = 4), histoplasmosis or
Irina Leonardi et al.
Cell host & microbe, 27(5), 823-829 (2020-04-17)
Fecal microbiota transplantation (FMT) targeting gut microbiota has recently been successfully applied to ulcerative colitis. However, only a subset of patients responds to FMT, and there is a pressing need for biomarkers of responsiveness. Fungi (the mycobiota) represent a highly
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实验方案

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

溶壁酶的酶学测定

本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。

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