生物来源
bovine muscle
质量水平
类型
Type X
检测方案
3.0—25.0 mg protein/mL (biuret)
形式
ammonium sulfate suspension
比活
≥600 units/mg protein
制造商/商品名称
Sigma-Aldrich
技术
activity assay: suitable
颜色
white
pH值（酸碱度）
7.5
应用
life science and biopharma
异质活性
pyruvate kinase ≤0.01%
储存温度
2-8°C
基因信息
cow ... LDHA(281274) , LDHB(281275)
一般描述
Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
应用
L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.
生化/生理作用
Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
单位定义
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
外形
Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0
分析说明
Protein determined by biuret.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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