L1378
L-Lactic Dehydrogenase 来源于牛肌肉
Type X, ammonium sulfate suspension, ≥600 units/mg protein
别名:
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
生物来源
bovine muscle
质量水平
类型
Type X
方案
3.0—25.0 mg protein/mL (biuret)
表单
ammonium sulfate suspension
比活
≥600 units/mg protein
制造商/商品名称
Sigma-Aldrich
技术
activity assay: suitable
颜色
white
pH值（酸碱度）
7.5
应用
life science and biopharma
异质活性
pyruvate kinase ≤0.01%
储存温度
2-8°C
基因信息
cow ... LDHA(281274), LDHB(281275)
一般描述
Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.
应用
L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.
生化/生理作用
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.
外形
Crystalline suspension in 2.4 M (NH4)2SO4 solution, pH 6.0
分析说明
Protein determined by biuret.
其他说明
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Metabolic recovery from submaximal exercise in hypoxia acclimated high altitude deer mice (Peromyscus maniculatus)
Dessureault LM, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 274, 111004-111004 (2024)
Biochemistry, lactate dehydrogenase
Farhana A and Lappin SL
StatPearls [Internet] (2020)
Deshdeepak Sahni et al.
Journal of neurosurgery. Pediatrics, 11(5), 575-583 (2013-03-12)
Graphene possesses unique electrical, physical, and chemical properties that may offer significant potential as a bioscaffold for neuronal regeneration after spinal cord injury. The purpose of this investigation was to establish the in vitro biocompatibility of pristine graphene for interface
D Steffens et al.
Journal of biomedical nanotechnology, 9(4), 710-718 (2013-04-30)
The association of stem cells (SCs) with biomaterials promises to be the protagonist for future regenerative medicine in the treatment of tissue and organ lesions. Stem cells were cultivated in scaffolds constructed by the electrospinning technique, using poly-D,L-lactic acid (PDLLA)
Minh Vu Chuong Nguyen et al.
Biochemical pharmacology, 85(11), 1644-1654 (2013-04-16)
NADPH oxidase Nox4 is expressed in a wide range of tissues and plays a role in cellular signaling by providing reactive oxygen species (ROS) as intracellular messengers. Nox4 oxidase activity is thought to be constitutive and regulated at the transcriptional
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
相关内容
QC Methods
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