KiCqStart 探针检测试剂盒

概述
预先设计的KiCqStart探针检测试剂盒可通过RT-qPCR（逆转录实时定量PCR）定量检测基因表达。为了符合 MIQE，该产品经复杂的生物信息学工具开发，并在计算机上进行了验证，以避免脱靶扩增¹KiCqStart探针检测试剂盒有三组适用于普通模式生物基因的正向引物、反向引物和双标记探针。

¹在设计过程中尽可能避免使用 SNP 位点。请咨询 dbSNP，以确定您的基因是否具有不可避免的 SNP。

可访问我们的常见问题部分，了解更多有关KiCqStart探针检测试剂盒的信息。

产品规格
设计特点

• GC含量(%)20 - 80%²
• 熔解温度：58.0 °C²
• 二级结构
• 过量 3′ 引物钳制最低
• 扩增子大小：75 – 150 bp
• 检测到剪切变体：最普遍

寡核苷酸特性（引物）

• 小分子：DNA（所有序列均已通过质谱QC验证）
• 内部修饰：
• 内容物：1 个正向引物和 1 个反向引物
• 长度：18 - 27 bp²
• 纯化方法：RP滤芯
• 形式：最少产生3 OD，干燥；2管，各含有1种寡核苷酸

寡核苷酸特性（探针）

• 小分子：DNA（所有序列均已通过质谱QC验证）
• 内部修饰：
• 5′荧光报告基团：6-FAM 或HEX

产品用于：基因表达分析

Product Specifications
Design Characteristics

• Exon-exon junction targeting: Assays are designed to span exon-exon junctions whenever possible, minimizing the risk of amplifying genomic DNA rather than the intended mRNA target. In cases where a suitable junction design is not available, a coding-region design is used as a fallback.
• SNP-aware design: Known single nucleotide polymorphism (SNP) positions are excluded during primer and probe design, so assays avoid sites of common genetic variation. This helps ensure consistent performance across samples from different individuals.
• Optimized amplicon size: Amplicons range from 75-200 bp, with most final assays falling between 75-150 bp, a size range well-suited for efficient, specific qPCR amplification.
• Transcript (isoform) coverage: Assays are designed to capture as many known transcript variants of a gene as possible.
• Secondary-structure screening: Probe sequences are computationally screened for a tendency to fold into secondary structures (e.g., hairpins) that could interfere with binding. Probes are filtered based on thermodynamic stability calculations to favor those less likely to form these structures.

Oligonucleotide Characteristics: Primer

• Molecule: DNA
• Purification: Desalt

Oligonucleotide Characteristics: Dual-LabeledProbe

• Molecule: DNA
• 5′ Reporter Dye: 6-FAM^™or HEX^™
• 3′ Quencher: Minor Groove Binder / Eclipse Dark Quencher (MGB:EDQ)
• Purification: HPLC

Product Format

• Components: Forward primer, reverse primer, and MGB:EDQ probe, available individual tubes or pre-mixed in a single clear tube.
• Coverage: All protein-coding genes.
• Shipping format: Sizes include – small (individual tubes, dry: ~2,200 rxn or pre-mixed in one tube dry @20X: 1,000 rxn), medium (individual tubes, dry: ~3,700 rxn), or large (individual tubes, dry: ~7,500 rxn).
• Storage temperature: −20 °C

KiCqStart^®探针检测试剂盒灵敏度高，具有特异性，省去了开发基于探针的有效RT-qPCR测定方法所需的繁琐步骤，可为您节省宝贵的时间和成本。该探针采用Sigma的暗 Onyx淬灭剂^™ (OQ^™)来标记，由于后者发热而不发光，信噪比优于传统的荧光淬灭剂。

优势包括：

• 已完成标靶分析
• 已指定设计特点
• 已指定寡核苷酸特点
• 订购方便

How to Order

1. Search by species and your gene of interest by gene name, gene symbol, or RefSeq accession number using the assay search tool above.
2. Select your assay and format from the results.
3. Add to cart.

For bulk orders, custom panel requirements, or assistance with gene target selection, please contact your local Merck representative.

6-FAM and HEX are trademarks of ThermoFisher Scientific.

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

HEX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries