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product name
Iscove 改良杜氏培养基, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
形式
powder
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
组分
phenol red: yes
glucose: yes
L-glutamine: yes
运输
ambient
储存温度
2-8°C
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一般描述
Iscove改良杜氏培养基是杜氏改良Eagle培养基(DME)的改良版本，含有硒、额外的氨基酸和维生素、丙酮酸钠、4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液、以及硝酸钾（代替硝酸铁）。据报道，Iscove培养基可支持鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和多种杂交细胞的生长。
应用
Iscove改良杜氏培养基已用于KG1a细胞（人白血病细胞株）和中国仓鼠卵细胞(CHO)DG44细胞株的培养。
据报道，其可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞的生长。
数量
配制成每升培养基中含 17.7 克粉末。
重悬
补充 3.024 g/L 碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Yogesh Kumar Verma et al.
International journal of radiation biology, 96(5), 671-688 (2020-01-28)
Purpose: Lithium chloride (LiCl) is clinically used for manic disorders. Its role has been shown in improving cell survival by decreasing Bax and p53 expression and increasing Bcl-2 concentration in the cell. This potential of LiCl is responsible for reducing
Ryan S Pawell et al.
Biomicrofluidics, 7(5), 56501-56501 (2014-01-10)
Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a
Elizabeth A Henderson et al.
Virology journal, 17(1), 43-43 (2020-04-03)
Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked
T Yoshikawa et al.
Cytotechnology, 33(1-3), 37-46 (2008-11-13)
In order to establish an easy and quick construction method for obtaining a stable and highly productive gene-amplified recombinant Chinese Hamster Ovary (CHO) cell line, variouskinds of stepwise methotrexate (MTX) selection were carriedout. The specific growth and production rates of
Hale M Tasyurek et al.
Human gene therapy, 29(7), 802-815 (2018-02-08)
Postprandial glucose-induced insulin secretion from the islets of Langerhans is facilitated by glucagon-like peptide-1 (GLP-1)-a metabolic hormone with insulinotropic properties. Among the variety of effects it mediates, GLP-1 induces delta cell secretion of somatostatin, inhibits alpha cell release of glucagon
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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