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产品名称
StableCell™ IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
杂质
Endotoxin, tested
组分
NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes
运输
ambient
储存温度
2-8°C
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一般描述
该 IMDM 与原处方不同，因为其添加了 L-丙氨酰-L-谷氨酰胺二肽。众所周知，谷氨酰胺在细胞培养中是不稳定的。这种二肽为细胞培养提供了一种更稳定的谷氨酰胺形式。
应用
经 Guilbert 和 Iscove 证明，红细胞和巨噬细胞的前体细胞可在添加白蛋白、转铁蛋白、卵磷脂和硒的完全确定的无血清培养基中培养。该培养基是对 Dulbecco 改良 Eagle 培养基 (DME) 的改良，含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES 缓冲液和硝酸钾而不是硝酸铁。进一步研究证明，Iscove 培养基可支持小鼠 B 淋巴细胞、骨髓造血组织、脂多糖刺激的 B 细胞、T 淋巴细胞和多种杂交细胞。
法律信息
StableCell is a trademark of Sigma-Aldrich Co. LLC
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Eleni Gelali et al.
Nature communications, 10(1), 1636-1636 (2019-04-11)
DNA fluorescence in situ hybridization (DNA FISH) is a powerful method to study chromosomal organization in single cells. At present, there is a lack of free resources of DNA FISH probes and probe design tools which can be readily applied.
Gustav Nilsonne et al.
Behavioral and brain functions : BBF, 7, 47-47 (2011-11-22)
Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus)
Christine Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
A model of chemical thymectomy by inducible Rag ablation was used to study peripheral T cell homeostasis. Induction of Rag ablation was efficient and complete, leading to cessation of thymic T cell production within 3-4 weeks. The decay of peripheral
Selective accumulation of aggregation-prone proteasome substrates in response to proteotoxic stress.
Florian A Salomons et al.
Molecular and cellular biology, 29(7), 1774-1785 (2009-01-23)
Conditions causing an increase in misfolded or aberrant proteins can impair the activity of the ubiquitin/proteasome system (UPS). This observation is of particular interest, given the fact that proteotoxic stress is closely associated with a large variety of disorders. Although
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