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Merck
CN

I1146

R3 IGF-1 人

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

IGF-1蛋白, 人IGF-1, 重组IGF-1

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关于此项目

NACRES:
NA.77
UNSPSC Code:
51111800

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产品名称

R3 IGF-1 人, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥85% (HPLC)

form

lyophilized powder

potency

≤10 ng/mL ED50

quality

endotoxin tested

mol wt

7,676 Da

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P05019

storage temp.

2-8°C

Quality Level

200

Gene Information

human ... IGF1(3479)

相关类别

Application

R3 IGF-I 是胰岛素样生长因子的重组类似物，包含完整的人 IGF-I 氨基酸序列，其中 Arg 取代了 Glu3。它被开发为一种廉价、高质量、高效的 IGF 类似物，可用作无血清或低血清细胞培养的生长因子补充剂。

使用专利表达系统在大肠杆菌中重组生产，该肽是从溶解并脱盐的包含体的细胞中收获。活性分子在氧化条件下适当折叠，然后通过多个液相色谱步骤纯化。

Biochem/physiol Actions

中国仓鼠卵细胞(CHO)的增殖。

Other Notes

胰岛素样生长因子-I 的重组类似物，在第 3 位用 Arg 取代 Glu。

Preparation Note

由0.1 M醋酸制备

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

兴奋剂肽类激素
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000446231
0000446231
0000422248
0000422248
0000352239

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?

    The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Insulin-like Growth Factors (IGF)

Insulin-like Growth Factors (IGF)

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