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质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no
运输
ambient
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一般描述
Hanks′平衡盐溶液的基本功能是维持pH值和渗透平衡。它还为细胞提供水和必需的无机离子。
配方：点击此处。
配方：点击此处。
应用
Hanks平衡盐溶液已被用于：
- 研究复合材料的降解和细胞毒性作用以及离子和/或磁铁矿从支架中的释放。
- 测量磁性支架材料在骨组织工程中的稳定性。
- 细胞内钙离子水平[Ca2+]的荧光测量
- 从脐带血中分离多形核白细胞（PMN）
- 评估小鼠的气道炎症
其他说明
酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于低密度的细胞培养。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
N Bock et al.
Acta biomaterialia, 6(3), 786-796 (2009-10-01)
In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the
A Tampieri et al.
Nanotechnology, 22(1), 015104-015104 (2010-12-08)
Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material
Iliana Moreschi et al.
The Journal of biological chemistry, 281(42), 31419-31429 (2006-08-24)
Micromolar concentrations of extracellular beta-NAD+ (NAD(e)+) activate human granulocytes (superoxide and NO generation and chemotaxis) by triggering: (i) overproduction of cAMP, (ii) activation of protein kinase A, (iii) stimulation of ADP-ribosyl cyclase and overproduction of cyclic ADP-ribose (cADPR), a universal
Janhavi Sharma et al.
Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)
One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase
Percíllia Victória Santos de Oliveira et al.
Redox biology, 22, 101142-101142 (2019-03-15)
Redox-related plasma proteins are candidate reporters of protein signatures associated with endothelial structure/function. Thiol-proteins from protein disulfide isomerase (PDI) family are unexplored in this context. Here, we investigate the occurrence and physiological significance of a circulating pool of PDI in
实验方案
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
人心脏成纤维细胞(HCF)分离自成人心室，用于研究心肌梗死、心脏纤维化和心脏肥大。它在细胞外基质维持、生长因子合成和细胞因子合成中起重要作用。本方案概述了人心脏成纤维细胞培养盒传代培养的推荐操作程序。
将细胞放入冻存管后须立即储存在液氮储罐中。
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