推荐产品
等级
BioPerformance Certified
质量水平
无菌性
0.2 μm filtered
形式
liquid
浓度
1 M
技术
cell culture | mammalian: suitable
杂质
Bioburden, tested
DNase, RNase, Protease, Nickase, free
endotoxin, tested
pH值（酸碱度）
5.0-6.0
有效pH范围
6.8-8.2
痕量阳离子
Fe: <5 ppm
heavy metals (as Pb): <5 ppm
适用性
suitable for molecular biology
应用
diagnostic assay manufacturing
SMILES字符串
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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一般描述
HEPES被认为是生物研究中最好的通用缓冲液之一。在生物酸碱度下，分子是两性离子，在酸碱度为6.8至8.2时可有效地作为缓冲剂。HEPES已被广泛应用，包括组织培养。它通常用于缓冲空气中的细胞培养基。研究发现HEPES在体外镁实验中亦可应用。
应用
核糖核酸酶HEPES已被用于：
- 补充Dulbecco“改良鹰”培养基以维持细胞系和RPMI培养基洗涤大鼠胰岛
- 回收纯化的核糖核苷酸
- 作为用于小核糖核酸分离和测序的HEPES/KOH缓冲液和腺苷酸化缓冲液的组分
- 作为用于核提取物制备的缓冲液中的一种成分，还可作为RPMI-1640培养基的添加剂，用于胰岛的维持
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
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