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H3537

Sigma-Aldrich

羟乙基哌嗪乙硫磺酸 溶液

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

别名:

N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)

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About This Item

CAS号:
7365-45-9
MDL编号:
MFCD00006158
UNSPSC代码:
12161700
PubChem化学物质编号:
329815199
NACRES:
NA.25

产品名称

羟乙基哌嗪乙硫磺酸 溶液, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

等级

BioPerformance Certified

质量水平

200

无菌性

0.2 μm filtered

表单

liquid

浓度

1 M

技术

cell culture | mammalian: suitable

杂质

Bioburden, tested
 DNase, RNase, Protease, Nickase, free
 endotoxin, tested

pH值（酸碱度）

5.0-6.0

有效pH范围

6.8-8.2

痕量阳离子

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

适用性

suitable for molecular biology

应用

diagnostic assay manufacturing

SMILES字符串

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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相关类别

一般描述

HEPES被认为是生物研究中最好的通用缓冲液之一。在生物酸碱度下，分子是两性离子，在酸碱度为6.8至8.2时可有效地作为缓冲剂。HEPES已被广泛应用，包括组织培养。它通常用于缓冲空气中的细胞培养基。研究发现HEPES在体外镁实验中亦可应用。

应用

核糖核酸酶HEPES已被用于：
  • 补充Dulbecco“改良鹰”培养基以维持细胞系和RPMI培养基洗涤大鼠胰岛
  • 回收纯化的核糖核苷酸
  • 作为用于小核糖核酸分离和测序的HEPES/KOH缓冲液和腺苷酸化缓冲液的组分
  • 作为用于核提取物制备的缓冲液中的一种成分，还可作为RPMI-1640培养基的添加剂，用于胰岛的维持

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000307185
0000307185
0000394584
0000394584
0000394585

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A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
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