H0887
羟乙基哌嗪乙硫磺酸 溶液
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
别名:
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
产品名称
羟乙基哌嗪乙硫磺酸 溶液, 1 M, sterile-filtered, BioReagent
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
sterility
sterile-filtered
product line
BioReagent, BioXtra
assay
0.9—1.1 M
form
liquid
storage condition
(Tightly closed. Dry), dry at room temperature
concentration
1 M
Quality Level
impurities
endotoxin, tested
color
colorless
useful pH range
6.8-8.2
7.5
density
1.1 g/cm3
suitability
suitable for component for culture media, suitable for electrophoresis
application(s)
cell analysis
life science and biopharma
sample preparation
storage temp.
2-8°C
正在寻找类似产品? 访问 产品对比指南
General description
1M的HEPES缓冲液（4-羟乙基哌嗪乙磺酸）经过无菌过滤处理，适于用作细胞生物学、生物化学和生物学研究中重要的生物缓冲液。HEPES是一种两性离子缓冲液，在pH 6.8-8.2范围内有效，适合丰富的研究领域。HEPES可用作细胞培养基的缓冲剂，相较Tris和磷酸盐有多项优势。尤其是它的细胞毒性更低，可在实验过程中维持最佳的细胞健康和活力。
此外，它可维持细胞生理pH，保护细胞免受呼吸引起的二氧化碳波动的影响，保证细胞稳态。这项能力更胜碳酸氢盐缓冲液(NaHCO3)。除了这些适合细胞培养的优异特性，HEPES也适于各种生物学和生物化学研究。也可用于免疫沉淀、细胞裂解、活细胞成像等技术，提供稳定受控的实验环境。功能多样、pH缓冲能力出色、与其他分子的互作力低，HEPES是多个研究领域中不可或缺的工具。
此外，它可维持细胞生理pH，保护细胞免受呼吸引起的二氧化碳波动的影响，保证细胞稳态。这项能力更胜碳酸氢盐缓冲液(NaHCO3)。除了这些适合细胞培养的优异特性，HEPES也适于各种生物学和生物化学研究。也可用于免疫沉淀、细胞裂解、活细胞成像等技术，提供稳定受控的实验环境。功能多样、pH缓冲能力出色、与其他分子的互作力低，HEPES是多个研究领域中不可或缺的工具。
它在细胞培养中常用的浓度为5mM到30mM。HEPES已被广泛用于各种应用，包括组织培养。它通常用于缓冲与空气接触的细胞培养基。HEPES可用于Mg的 体外 实验。
Application
HEPES可作为组分：
- 配制Hank’平衡盐溶液、Dulbecco’改良eagle’培养基和无糖DMEM，用于制备组织切片
- 配制缓冲液，用于细胞培养小室底侧的局部电导测量，以检测阳离子选择性
- 配制细胞培养基，提供缓冲能力维持细胞株pH
- 配制缓冲液，用于猪髓核的脱细胞反应
Features and Benefits
- 高纯度产品，适合生化和生物学研究
- 除菌过滤处理，并通过细胞培养测试
- 即用型溶液
- 适用pH范围：6.8 - 8.2，pKa 7.5(25 °C)
- 即用型溶液，无需配制，节省时间
Preparation Note
由HEPES游离酸和细胞培养级水制备。
Disclaimer
浓度超过40mM的HEPES对某些细胞类型可能会有毒性。
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Kaixi Tang et al.
PLoS computational biology, 14(7), e1006303-e1006303 (2018-07-06)
Focal adhesions are protein complexes that anchor cells to the extracellular matrix. During migration, the growth and disassembly of these structures are spatiotemporally regulated, with new adhesions forming at the leading edge of the cell and mature adhesions disassembling at
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland Nt
Acta Biomaterialia, 8, 925- 936 (2012)
Zuzana Keckesova et al.
Nature, 543(7647), 681-686 (2017-03-23)
Post-mitotic, differentiated cells exhibit a variety of characteristics that contrast with those of actively growing neoplastic cells, such as the expression of cell-cycle inhibitors and differentiation factors. We hypothesized that the gene expression profiles of these differentiated cells could reveal
The role of the endocrine system in feeding-induced tissue-specific circadian entrainment.
Sato M, et al.
Cell Reports, 8(2), 393-401 (2014)
Stereological monitoring of mouse lung alveolarization from the early postnatal period to adulthood.
Agnieszka Pozarska et al.
American journal of physiology. Lung cellular and molecular physiology, 312(6), L882-L895 (2017-03-21)
Postnatal lung maturation generates a large number of small alveoli, with concomitant thinning of alveolar septal walls, generating a large gas exchange surface area but minimizing the distance traversed by the gases. This demand for a large and thin gas
相关内容
Product Information Sheet
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持