生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~36 kDa
种属反应性
mouse, rat, human
浓度
~1 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates
indirect immunofluorescence: 5-10 μg/mL using rat NRK cells
western blot: 0.1-0.2 μg/mL using whole extract of human HeLa cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)
一般描述
特异性
免疫原
应用
- 人癌细胞提取物
- 少肌症老年小鼠肌肉组织提取物
- 骨小梁或小鼠心肌样本
- 小鼠胚胎成纤维细胞
生化/生理作用
外形
储存及稳定性
想要延长储存,工作分装冷冻。不建议反复冻融或存放在无霜冰柜中。如果长期储存时出现轻微浑浊,请在使用前通过离心澄清溶液。工作稀释样本如果在12小时内没有使用,应该丢弃。
免责声明
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WGK
WGK 1
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
商品
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Loading controls in western blotting application.
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
本页面介绍了一篇有关瓦博格效应的文章,以及其如何能够在正常氧气水平下,增强肿瘤细胞中葡萄糖向乳酸的转化。Otto Heinrich Warburg在1924年证明,癌细胞显示出对糖酵解的依赖性增加,以满足他们的能量需求,无论是否有充足的氧气存在。
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