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一般描述
Gamborg′s B-5基础培养基是一种有效的植物营养混合物。
Gamborg′B-5培养基可以完全培养基（G5893）进行购买或通过从Vitamin 1000x（G1019）和Basal Salt混合物（G5768）等化学品储备物进行复溶而得。使用Gamborg′B-5（B5）培养基用于植物细胞系和组织的体外培养，如墨西哥红豆杉、大壶藓、白亚麻、赤桉。Gamborg′B-5培养基已被用于培养番荔枝愈伤组织。
应用
Gamborg′s B-5植物组织基础培养基（含微量有机物）还用于：
- 作为小白菜(Brassica rapa)组织液体培养基组分
- 作为愈伤诱导培养基组分，培养拟南芥心磷脂突变幼苗
- 配成固体培养基，用于拟南芥种子发芽
公式变量
数量
配制成每升培养基含 3.2 克粉末。
警示用语：
Warning
危险声明
危险分类
Ox. Sol. 3
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
危险化学品
Experiments in Plant Tissue Culture (1985)
Leigh Mickelson-Young et al.
Journal of experimental botany, 67(21), 6077-6087 (2016-11-05)
The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on
Martin E Kolewe et al.
Plant cell reports, 29(5), 485-494 (2010-03-11)
Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus
Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
商品
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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