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G1N350

Sigma-Aldrich

GenElute™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 350 purifications

别名:

哺乳动物基因组DNA小量制备, Gen Elute

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About This Item

UNSPSC代码:
41105501
NACRES:
NA.55

用途

sufficient for 350 purifications

质量水平

200

技术

DNA purification: suitable

储存温度

15-25°C

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相关类别

一般描述

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

可从培养细胞、组织（包括啮齿动物尾巴）以及新鲜的全血或白血细胞之中分离高纯度的基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

应用

纯化所得的基因组DNA可直接供以下下游应用使用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆
  • 连接
GenElute™ Mammalian Genomic DNA Miniprep Kits has been used:
  • to extract DNA from fin samples
  • to extract the BSTUI restriction enzyme from the DNA
  • to isolate DNA from the blood samples extracted from caudal fin
  • to extract tissue and hair follicle DNA

特点和优势

  • 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
  • 洗脱体积：200 - 400 μl
  • 所需时间：细胞裂解后20分钟
  • A260/A280比率：1.6 - 1.9
  • 是否需要机械式均质化：否

原理

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

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警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000342924
0000344637
0000342924
0000344637
SLCR2891

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Gudrun Dittrich-Schröder et al.
Molecular ecology resources, 12(1), 109-115 (2011-09-29)
DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of
Genetic shifts in the transition from wild to farmed white-tailed deer (Odocoileus virginianus) population
Hernandez-Mendoza P M, et al.
International Journal of Biodiversity Science, Ecosystem Services & Management, 10(1), 3-8 (2014)
Novel single nucleotide polymorphisms in candidate genes for growth in tilapia (Oreochromis niloticus)
Cuevas-Rodriguez B L, et al.
Revista Brasileira de Zootecnia, 45(6), 345-348 (2016)
Takahiro Seki et al.
Journal of controlled release : official journal of the Controlled Release Society, 156(3), 381-389 (2011-09-03)
Tumour Necrosis Factor alpha (TNF) is a pleiotropic pro-inflammatory cytokine with known vascular permeabilising activity. It is employed during isolated limb perfusion to enhance delivery of chemotherapeutic drugs into tumour tissue. The use of conditionally-replicating lytic viruses, so called 'oncolytic
Apollo Simon Peter Balyeidhusa et al.
Veterinary parasitology, 187(1-2), 157-167 (2012-01-17)
The role played by domestic animals in the transmission of gambiense Human African Trypanosomosis remains uncertain. Northwest Uganda is endemic for Trypanosoma brucei gambiense. Of the 3267 blood samples from domestic animals in four counties examined by hematocrit centrifugation technique
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实验方案

GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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相关内容

Mammalian Genomic DNA Miniprep Kit

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

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