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Merck
CN
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安全信息

G1279

Sigma-Aldrich

G 418 二硫酸盐

powder, suitable for plant cell culture, BioReagent

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别名:
抗生素G418
经验公式（希尔记法）:
C20H40N4O10 · 2H2SO4
CAS号:
108321-42-2
分子量:
692.71
MDL编号:
MFCD00058314
UNSPSC代码:
10171502
PubChem化学物质编号:
24895058
NACRES:
NA.72

产品线

BioReagent

质量水平

200

形式

powder

效能

≥720 μg per mg (Dried basis)

技术

cell culture | plant: suitable

颜色

white to off-white

溶解性

100 mg/mL (H2O clear)

抗生素抗菌谱

Gram-negative bacteria

应用

agriculture

作用机制

protein synthesis | interferes

储存温度

2-8°C

SMILES字符串

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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相关类别

一般描述

Chemical structure: aminoglycoside

应用

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

注意

The product is stable for three years when stored at 2-8°C.

制备说明

G418 powder is soluble in water at 100 mg/mL.

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334

危险分类

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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Luis M Molinos-Albert et al.
Retrovirology, 11, 44-44 (2014-06-10)
The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and
Frida C Mohlin et al.
Molecular immunology, 65(2), 367-376 (2015-03-04)
Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study
Glenda Eger et al.
PloS one, 9(9), e109047-e109047 (2014-10-01)
The fibroblast mitogen platelet-derived growth factor -BB (PDGF-BB) induces a transient expression of the orphan nuclear receptor NR4A1 (also named Nur77, TR3 or NGFIB). The aim of the present study was to investigate the pathways through which NR4A1 is induced
Yan-Ping Li et al.
STAR protocols, 2(2), 100438-100438 (2021-04-27)
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR)
Elsa A Marquez et al.
PloS one, 10(7), e0131308-e0131308 (2015-07-07)
Ly49 receptors can be peptide selective in their recognition of MHC-I-peptide complexes, affording them a level of discrimination beyond detecting the presence or absence of specific MHC-I allele products. Despite this ability, little is understood regarding the properties that enable
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