G0413
β(1→4)-半乳糖苷酶，位置特异性 来源于肺炎链球菌
recombinant, expressed in E. coli, buffered aqueous solution
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥6 units/mg protein
Recombinant:
expressed in E. coli
Quality Level
recombinant
expressed in E. coli
form
buffered aqueous solution
specific activity
≥6 units/mg protein
packaging
vial of 0.06 unit
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... GLB1(2720)
General description
β-半乳糖苷酶存在于细菌、真菌、酵母和动物器官中。也存在于苹果、杏仁和杏等水果中。β-半乳糖苷酶是四聚体，由四条多肽链组成，这四条多肽链由氨基酸组装形成五个结构域。这些区域为果冻卷饼桶型，中心区域作为活性位点，其余区域由 β-夹心结构和纤连蛋白组成。
Application
β（1→4）-半乳糖苷酶，是 肺炎链球菌 的位置特异性酶，可用于：
- 作为位置特异性酶，在小鼠中研究其末端半乳糖基化作用以及对糖鞘脂（GSPL）的保护作用。
- 用于消化放射性低聚糖。
- 作为位置特异性酶，研究其对无毒 杜氏利什曼原虫 克隆（A-LD）毒力特性的影响。
Biochem/physiol Actions
β-半乳糖苷酶可水解低聚糖、聚合物和次级代谢产物中的 D-半乳糖基部分。广泛用于乳制品行业，用于去除牛奶和乳制品中的乳糖，使乳糖不耐受的个体获益。β-半乳糖苷酶也可用于食品行业，改善甜度、口感和溶解情况。
Physical form
溶于20 mM Tris-HCl，pH 7.5、25 mM NaCl的溶液中
Other Notes
在pH 5.0、37 °C下，一个单位每分钟可水解1 μmole的对硝基苯基β-D-吡喃半乳糖苷。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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