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Merck
CN

F9037

甲酰胺

≥99.5% (GC), BioReagent, Molecular Biology

别名:

C1 酰胺, 氨基甲醛

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关于此项目

线性分子式:
HCONH2
化学文摘社编号:
75-12-7
分子量:
45.04
UNSPSC Code:
12352111
NACRES:
NA.52
PubChem Substance ID:
24894985
EC Number:
200-842-0
Beilstein/REAXYS Number:
505995
MDL number:
MFCD00007941
Grade:
Molecular Biology
Assay:
≥99.5% (GC)
Technique(s):
electrophoresis: suitable
immunohistochemistry: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

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产品名称

甲酰胺, ≥99.5% (GC), BioReagent, Molecular Biology

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

SMILES string

NC=O

grade

Molecular Biology

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

BioReagent

assay

≥99.5% (GC)

form

liquid

autoignition temp.

932 °F

technique(s)

electrophoresis: suitable
immunohistochemistry: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

suitability

suitable for nucleic acid hybridization

storage temp.

2-8°C

Quality Level

200

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Application

  • 核酸杂交
  • 防冻剂和凝胶稳定剂
  • 弱化核酸双链体稳定性
  • 消除核酸二级结构
  • 制备RNA和DNA应用所需的杂交缓冲液
甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。
甲酰胺适用于使用标准配方配制的杂交缓冲液。甲酰胺可弱化核酸双链体稳定性，通常在需要较低杂交温度的杂交方案中50％浓度使用。

其他应用包括：

  • 制备用于BrdU免疫组化的小鼠脑切片
  • HeLa细胞FISH分析。
  • 使用变性梯度凝胶电泳法制备用于PCR产物分析的聚丙烯酰胺凝胶（《The Merck Index》第12版）。

Features and Benefits

  • 分子生物学应用去离子
  • 封装在氩气填充的棕色瓶中，以防氧化
  • 用于DNA复性或DNA-RNA杂交
  • 可降低双链核酸的热稳定性

General description

甲酰胺是一种衍生自甲酸的透明液体酰胺。甲酰胺可让核酸在室温下变性和复性。

Other Notes

该产品针对分子生物学应用所需去离子化，包装在氩气填充棕色玻璃瓶中防止氧化。

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000511146
0000511146
0000508499
0000508499
0000481619

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Laurent Geiser et al.
Journal of chromatography. A, 979(1-2), 389-398 (2002-12-25)
A nonaqueous capillary electrophoresis (NACE) method, coupled with either UV or electrospray mass spectrometry (ESI-MS), is described for the simultaneous analysis of seven beta-blockers. The same electrolyte, namely 25 mM ammonium formate and 1 M formic acid, was used with
T Kaabache et al.
Analytical biochemistry, 232(2), 225-230 (1995-12-10)
The sensitivity of direct solution hybridization of hepatocytes solubilized in guanidium thiocyanate (GuSCN) for detecting alpha 1-acid glycoprotein and albumin mRNAs was studied. The sensitivity of detection was inversely correlated with the DNA concentration. Raising the hybridization temperature from 20
N C Pagratis
Nucleic acids research, 24(18), 3645-3646 (1996-09-15)
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C
Jianwei Li
Journal of pharmaceutical sciences, 91(8), 1873-1879 (2002-07-13)
This article describes the proper selection of extraction solvents to eliminate interference from a polymer matrix to the quantitation of estradiol degradation products in a transdermal formulation by reversed-phase liquid chromatography. The separation is performed by gradient elution with acetonitrile
Helena Mistry et al.
Genome integrity, 1(1), 7-7 (2010-08-04)
DNA double-strand breaks (DSBs) caused by ionizing radiation or by the stalling of DNA replication forks are among the most deleterious forms of DNA damage. The ability of cells to recognize and repair DSBs requires post-translational modifications to histones and
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Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

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