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安全信息

F0895

Sigma-Aldrich

Human Fibronectin

from human plasma, liquid, 1 mg/mL, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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About This Item

MDL编号:
MFCD00131062
UNSPSC代码:
12352202
eCl@ss:
42020141
NACRES:
NA.75

product name

人血浆纤维连接蛋白, liquid, 0.1% (Solution), BioReagent, suitable for cell culture

生物来源

human plasma

质量水平

200

无菌性

sterile

产品线

BioReagent

形式

liquid

包装

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

浓度

0.1% (Solution)

技术

cell culture | mammalian: suitable

表面覆盖度

1‑5 μg/cm2

溶解性

saline: 0.05 M (Tris-buffered)

UniProt登记号

P02751

结合特异性

Peptide Source: Collagen

运输

wet ice

储存温度

2-8°C

基因信息

human ... FN1(2335)

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相关类别

一般描述

纤连蛋白1是由FN1基因编码的细胞外基质糖蛋白。它在血浆和细胞表面表达。FN1 基因位于人染色体2q35。
FN 是一种二聚体蛋白，通过C末端的两个二硫键连接起来。在纤连蛋白结构内部，结构域在接近肽链的氨基和羧基末端的位置紧密排布。此外，研究人员观察到，尽管来源于单个基因，在人体中鉴定出了多达20个不同的基因变异体。

应用

纤连蛋白被应用于测定人血浆H2O2 的消耗速率。它也被用于包被细胞培养用Sylgard® 模型。它也被用于细胞黏附和将蛋白质直接印在聚合膜上。

生化/生理作用

纤连蛋白的功能涵盖各种生物过程，包括细胞粘附、生长、迁移、伤口愈合、凝血和转移。 纤连蛋白在细胞附着和扩散、细胞骨架控制、细胞形态和分化等方面发挥着重要作用。FN1还参与细胞外基质的形成、止血和血栓形成。FN1基因突变可导致肾小球病变。

特点和优势

  • 粘着糖蛋白
  • 来源于人血浆
  • 建议表面覆盖率为 1-5 μg/cm2

制备说明

本品为0.1%纤连蛋白溶液。该蛋白的均质性由免疫电泳检测，通过内毒素测试，其源材料HIV、乙肝、丙肝检测均为阴性。

在涂层培养表面，纤连蛋白应在无菌平衡的无菌盐溶液中稀释，并采用最小的涂层体积。 表面应在室温下风干45分钟，并可在2-8℃下保存2-4周

分析说明

蛋白质均一性由免疫电泳评估。

法律信息

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

免责声明

仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。

相关产品

产品编号
说明
价格

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should I store and handle Product F0895, Fibronectin from human plasma?

    Recommended storage of this material is  2-8 °C.  Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

  4. Is Product F0895, Fibronectin from human plasma, sterile?

    Fibronectin 0.1% solution is a sterile product.  It is tested for sterility.  The sterility test results can be found on the lot-specific certificate of analysis.

  5. What is the solvent for Product F0895, Fibronectin from human plasma?

    This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.

  6. How much Product F0895, Fibronectin from human plasma, do I use for my application?

    Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease.
Farcas M A, et al.
BioMedical Engineering OnLine, 8(1), 30-30 (2009)
Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation.
Altomare L, et al.
The International Journal of Artificial Organs, 33(8), 535-543 (2010)
Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.
Birkenkamp-Demtroder K, et al.
British Journal of Cancer, 105(4), 552-552 (2011)
Fibronectin: Molecular Structure, Fibrillar Structure and Mechanochemical Signaling
Dalton C J and Lemmon C A
Cell, 10(9), 2443-2443 (2021)
Ana Bagulho et al.
Redox biology, 6, 454-460 (2015-09-27)
The molecular processes that are crucial for cell function, such as proliferation, migration and survival, are regulated by hydrogen peroxide (H2O2). Although environmental cues, such as growth factors, regulate redox signaling, it was still unknown whether the ECM, a component
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