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安全信息

F0291

Sigma-Aldrich

hBFGF

FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

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别名:
成纤维细胞生长因子 7-碱性 人, FGF2, bFGF
CAS号:
106096-93-9
MDL编号:
MFCD00081382
UNSPSC代码:
12352202
NACRES:
NA.77

生物来源

human

质量水平

200

重组

expressed in E. coli

检测方案

≥97% (SDS-PAGE)

形式

lyophilized powder

效能

≤-0.6 ng/mL

分子量

16.0 kDa

包装

pkg of 4X25 μg
pkg of 25 μg

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

杂质

≤1.00 EU/μg

颜色

white to faint yellow cast

溶解性

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt登记号

P09038

储存温度

−20°C

基因信息

human ... FGF2 (2257)

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相关类别

一般描述

碱性成纤维细胞生长因子（FGF-Basic） 存在于基底膜和内皮下细胞外基质中。FGF-Basic 在胚胎发育早期被诱导，它是胚胎干细胞（ESC） 和谱系定义的干细胞的多能性和自我更新所必需的（但不充分）； 其中它起到支持增殖和抑制分化的作用。hESC 的自我更新取决于激活素/Nodal/Smad2,3 分支的激活和 TGFβ 家族细胞信号传导程序的 BMP/GDF/Smad5 分支的抑制。FGF-Basic 是多能性节点支持的感受态因子。FGF-Basic 是维持干细胞特性所必需且充分的。它诱导人 ES 细胞中 Nodal 和 FGF-Basic 的表达。

应用

碱性成纤维细胞生长因子（FGF-Basic）是维持培养物中人胚胎干细胞和各种其它干细胞系，如间充质干 （基质） 细胞 （MSC） 所必需的。

外形

从 20 mM Tris、1 M NaCl（pH 7.0，每1 μg BFGF含 50μg 牛血清白蛋白 (BSA)/1μg 脂联素）的 0.2 μm 过滤溶液冻干。

分析说明

FGF-Basic 的生物活性是在荧光测定中使用氧化还原敏感型染料刃天青进行测量。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Brad Davidson et al.
Genes & development, 20(19), 2728-2738 (2006-10-04)
Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for characterizing complex developmental processes, such as the initial phases of chordate heart development. The basic helix-loop-helix regulatory gene Ci-Mesp is required for activation of cardiac transcription factors. Evidence is
R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Structural characterization and biological functions of fibroblast growth factor.
D Gospodarowicz et al.
Endocrine reviews, 8(2), 95-114 (1987-05-01)
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