跳转至内容
Merck
CN
所有图片(2)

主要文件

查看所有文档

F0291

Sigma-Aldrich

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

成纤维细胞生长因子 7-碱性 人, FGF2, bFGF

登录查看公司和协议定价
所有图片(2)

About This Item

CAS号:
106096-93-9
MDL编号:
MFCD00081382
UNSPSC代码:
12352202
NACRES:
NA.77

产品名称

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

生物来源

human

质量水平

200

重组

expressed in E. coli

方案

≥97% (SDS-PAGE)

表单

lyophilized powder

效能

≤-0.6 ng/mL

分子量

16.0 kDa

包装

pkg of 4X25 μg
pkg of 25 μg

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

杂质

≤1.00 EU/μg

颜色

white to faint yellow cast

溶解性

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt登记号

P09038

储存温度

−20°C

基因信息

human ... FGF2 (2257)

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

碱性成纤维细胞生长因子（FGF-Basic） 存在于基底膜和内皮下细胞外基质中。FGF-Basic 在胚胎发育早期被诱导，它是胚胎干细胞（ESC） 和谱系定义的干细胞的多能性和自我更新所必需的（但不充分）； 其中它起到支持增殖和抑制分化的作用。hESC 的自我更新取决于激活素/Nodal/Smad2,3 分支的激活和 TGFβ 家族细胞信号传导程序的 BMP/GDF/Smad5 分支的抑制。FGF-Basic 是多能性节点支持的感受态因子。FGF-Basic 是维持干细胞特性所必需且充分的。它诱导人 ES 细胞中 Nodal 和 FGF-Basic 的表达。

应用

碱性成纤维细胞生长因子（FGF-Basic）是维持培养物中人胚胎干细胞和各种其它干细胞系，如间充质干 （基质） 细胞 （MSC） 所必需的。

外形

从 20 mM Tris、1 M NaCl（pH 7.0，每1 μg BFGF含 50μg 牛血清白蛋白 (BSA)/1μg 脂联素）的 0.2 μm 过滤溶液冻干。

分析说明

FGF-Basic 的生物活性是在荧光测定中使用氧化还原敏感型染料刃天青进行测量。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000195944
0000372843
0000355296
0000195944
0000372843

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Brad Davidson et al.
Genes & development, 20(19), 2728-2738 (2006-10-04)
Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for characterizing complex developmental processes, such as the initial phases of chordate heart development. The basic helix-loop-helix regulatory gene Ci-Mesp is required for activation of cardiac transcription factors. Evidence is
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
Lei Xiao et al.
Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)
Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an
查看所有相关文献

商品

成纤维细胞生长因子家族(FGF)

用于细胞培养的成纤维细胞生长因子，以及用于您的研究的各类生长因子

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

查看所有结果

实验方案

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门