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Merck
CN

E6909

Sigma-Aldrich

ECM 凝胶 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤

growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture

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别名:
GFR Basement Membrane Extract (BME)
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

产品线

BioReagent

形式

liquid

浓度

7-9 mg/mL

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−20°C

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应用

ECM凝胶可用于上皮细胞、内皮细胞、肌肉细胞、神经细胞和肿瘤细胞。
来自Engelbreth-Holm-Swarm鼠肉瘤的ECM凝胶已被用于涂覆不同神经胶质瘤亚型和C2C12肌肉细胞的组织培养板。它还可被用作增加肌管附着、成熟和存活的支架。

生化/生理作用

当达到20-40°C时,ECM基质胶将发生热活化聚合,形成重组基底膜。 当PC12细胞在该ECM胶薄层上生长时,将在2天内形成神经突。

用于生产本品的每个小鼠种群都定期筛查病原体。经检测,LDEV呈阴性。

组分

ECM制剂的蛋白质浓度为8-12 mg/mL,主要成分为层粘连蛋白,还含有IV型胶原蛋白、蛋白多糖硫酸乙酰肝素、巢蛋白等其他次要成分。 向ECM凝胶中添加IV型胶原蛋白可增加其聚合度。
生长因子减少的修改可为培养基提供更多的控制,更好地解释结果并提高聚合度。 该培养基还缺少可能会干扰某些分析的酚红。

注意

如需长期储存,请将本品置于-20°C储存。ECM凝胶可在2-8℃下储存最长72小时。

制备说明

该产品是从小鼠Engelbreth-Holm-Swarm肉瘤中提取的;并经过氯仿透析。 使用前,将凝胶置于2-8℃下解冻过夜,并使用预冷至2-8℃的板和移液器将凝胶分配至多孔板。该凝胶中可能含有不会影响其活性的沉淀物。 该凝胶也可使用2-8℃的Dulbecco′改良Eagle′培养基将凝胶稀释最多两倍,应先稀释、再分配至多孔板。 该产品在20°C下放置5分钟即可成胶。对于长时间操作,应在低于10°C的温度下进行。细胞可接种于0.5 mm厚的凝胶层上或在1mm厚的凝胶层内培养。 如果在凝胶层内培养细胞,请在接种前将细胞加至凝胶中,建议密度为3-4 x 104 个细胞/mL。 可使用溶剂为PBS,不含钙、镁或EDTA且浓度为0.6-2.4单位/mL的蛋白酶,将细胞从凝胶中分离出来。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?

    Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.

  5. Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?

    Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.

  6. Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?

    Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product.  Product No. E6909, ECM gel, growth factor reduced, is phenol red free.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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