一般描述
抗生物素蛋白 (Avidin) 是一种四聚体或二聚体的生物素结合蛋白，产生于鸟类、爬行动物和两栖动物的输卵管中，并沉积在其蛋清中。由生物素的 4 个高亲和力结合位点组成。ExtrAvidin ® 由蛋清亲和素制备而成。它是亲和纯化亲和素的改良形式，具有亲和素的高比活性和链霉亲和素的低背景染色。是一种由 阿维迪链霉菌 产生的生物素结合蛋白。ExtrAvidin已与碱性磷酸酶偶联，可用于多种技术。
应用
ExtrAvidin ™-碱性磷酸酶已被用于：
- 斑点杂交法
- 免疫组化（福尔马林固定、石蜡包埋切片）
- 间接 ELISA
- 酶联免疫吸附试验 (ELISA)
- 酶联免疫吸附点 (ELISPOT) 分析
- 竞争性ELISA
生化/生理作用
碱性磷酸酶催化酯水解生成磷酸。 它还促进物质的跨膜运输。
外形
含有1 mM MgCl2、1% 牛血清白蛋白和15 mM 叠氮化钠的 0.05 M Tris-HCl 缓冲液，pH 8.0中的溶液
制备说明
亲和纯化蛋白
法律信息
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
W E Sweeney et al.
Kidney international, 56(2), 406-413 (1999-08-05)
Recessively transmitted polycystic kidney disease (PKD) in many murine models is characterized by the initial formation of proximal tubular cysts (stage 1), followed by growth and enlargement of renal collecting tubule (CT) cysts (stage 2). Previous studies have reported that
Joanne L Maki et al.
Clinical and diagnostic laboratory immunology, 10(5), 876-881 (2003-09-11)
Fish acquire protective immunity against the ciliated protozoan parasite Ichthyophthirius multifiliis following sublethal infection or inoculation with I. multifiliis immobilization antigens (i-antigens). In both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. To investigate the kinetics of
C E Rutten et al.
Leukemia, 22(7), 1387-1394 (2008-04-18)
Mismatching for human leukocyte antigen (HLA)-DPB1 in unrelated donor hematopoietic stem cell transplantation (URD-SCT) has been associated with a decreased risk of disease relapse, indicating that HLA-DP may represent a target for graft-versus-leukemia (GVL) reactivity in HLA class II-expressing hematological
Alkaline Phosphatase (2103)
Robin B Gore et al.
The Journal of allergy and clinical immunology, 117(3), 649-655 (2006-03-09)
The relationship among inhaled allergen exposure, sensitization, and asthma severity is unknown. To investigate the relationship among personal allergen exposure, reservoir dust allergen concentrations, and physiological measures of asthma severity; to examine the numbers of particles inspired that react with
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
实验方案
本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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