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应用
来自 和平空间站伊金氏菌 的内切糖苷酶 F3 已用于分析血清蛋白的核心岩藻糖基化和胰蛋白酶消化物。
生化/生理作用
内切糖苷酶 F3 属于糖苷水解酶家族 18 (GH18)。具有水解活性。内切糖苷酶 F3 糖基化 α-1,6-岩藻糖基化 GlcNAc 衍生物，得到天然核心岩藻糖基化复合物型 N -糖肽。
裂解天冬酰胺连接的双天线和三天线复合物，寡糖取决于核心岩藻糖基化和肽键的状态。
包装
提供 5× 反应缓冲液，250 mM 醋酸钠，pH 4.5
单位定义
一个单元将在37°C pH 4.5 下每分钟从 1μmole 变性猪纤维蛋白原释放 N-连接寡糖。
外形
20 mM Tris-HCl，pH 7.5中的无菌填充溶液
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Chemical Biology of Glycoproteins (2017)
Advances in Carbohydrate Chemistry (2016)
Liwei Cao et al.
Nature communications, 13(1), 3910-3910 (2022-07-08)
Core fucosylation of N-linked glycoproteins has been linked to the functions of glycoproteins in physiological and pathological processes. However, quantitative characterization of core fucosylation remains challenging due to the complexity and heterogeneity of N-linked glycosylation. Here we report a mass
Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM
Ma J, et al.
Journal of proteomics, 189, 67-74 (2018)
Characterization of novel endo-beta-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG
Huang Y, et al.
Scientific reports, 8(1), 246-246 (2018)
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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