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E1510

溴化乙啶溶液

Name: 溴化乙啶 溶液
Brand: SIGMA
Price: 1131.82 CNY
Availability: InStock

BioReagent, Molecular Biology, 10 mg/mL in H₂O

别名:

3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵

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100 G

¥1,131.82

500 G

¥2,695.24

1 KG

¥3,880.89

5 KG

¥17,464.65

10 KG

¥17,827.66

¥1,131.82

国内现货，预计发货时间2025年4月27日详情

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100 G

¥1,131.82

500 G

¥2,695.24

1 KG

¥3,880.89

5 KG

¥17,464.65

10 KG

¥17,827.66

About This Item

经验公式（希尔记法）:

C₂₁H₂₀BrN₃

CAS号:

1239-45-8

分子量:

394.31

Beilstein:

3642536

MDL编号:

MFCD00011724

UNSPSC代码:

41105322

PubChem化学物质编号:

24894418

NACRES:

NA.31

¥1,131.82

国内现货，预计发货时间2025年4月27日详情

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此商品	T3253	T15760	T6666
T5941 Trizma^® 盐酸盐	T3253 Trizma^® 盐酸盐	T15760 Trizma^® 盐酸盐	T6666 Trizma^® 盐酸盐
assay ≥99.0% (titration)	assay ≥99.0% (titration)	assay ≥99%	assay ≥99.0% (titration)
technique(s) DNA purification: suitable, electrophoresis: suitable, cell culture \| mammalian: suitable, protein extraction: suitable	technique(s) affinity chromatography: suitable	technique(s) -	technique(s) -
grade BioPerformance Certified	grade reagent grade	grade reagent grade	grade -
Quality Level 400	Quality Level 400	Quality Level 200	Quality Level 400
form crystalline powder	form crystalline	form crystalline	form powder
application(s) diagnostic assay manufacturing general analytical life science and biopharma sample preparation	application(s) diagnostic assay manufacturing life science and biopharma sample preparation	application(s) -	application(s) diagnostic assay manufacturing

应用

溴化乙啶 (EtBr) 为聚丙烯酰胺凝胶电泳或琼脂糖凝胶电泳中最常用的核酸染料。EtBr 在结合到双链 RNA 上时其荧光可提高 21 倍，在结合到双链 DNA 上可提高 25 倍，从而使得在低染料浓度 (10μg/ml) 下无需背景脱色。溴化乙啶已用于核酸的许多荧光分析。已表明它可结合到单链 DNA（虽然强度不高）和三链 DNA 上。由于 EtBr 能够结合到 DNA 上，因此它是 DNA 聚合酶的抑制剂。

溴化乙锭溶液可用于：

用作可视化U937细胞的染色剂，以评估细胞活性[1]
检测聚合酶链反应产物[2]
基于琼脂糖凝胶电泳的凝胶阻滞测定[3]

生化/生理作用

溴化乙啶可插入双链 DNA 和 RNA 中，并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

重悬

对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。

警示用语：

Danger

危险声明

H331,H341

预防措施声明

P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313

危险分类

Acute Tox. 3 Inhalation - Muta. 2

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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pH-sensitive oncolytic adenovirus hybrid targeting acidic tumor microenvironment and angiogenesis

Choi JW, et al.

Journal of Controlled Release : Official Journal of the Controlled Release Society, 205, 134-143 (2015)

Application of a new wall-less plate technology to complex multistep cell-based investigations using suspension cells

Quinones GA, et al.

Blood, 121(7), e25-e33 (2013)

Rapid DNA amplification using a battery-powered thin-film resistive thermocycler.

Keith E Herold et al.

Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)

A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling

Widespread intron retention in mammals functionally tunes transcriptomes.

Ulrich Braunschweig et al.

Genome research, 24(11), 1774-1786 (2014-09-27)

Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is

ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells.

Patrick Seitz et al.

PLoS genetics, 10(1), e1004066-e1004066 (2014-01-07)

The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculative. Here we

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The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

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溴化乙啶 溶液

BioReagent, Molecular Biology, 10 mg/mL in H2O

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溴化乙啶溶液

BioReagent, Molecular Biology, 10 mg/mL in H₂O