应用
来自Sigma的酶已用于激活胰蛋白酶原，以测量猪胰腺中胰蛋白酶的活性。研究表明，抗菌治疗可减少断奶猪肠道菌群，提高蛋白消化能力，而不会改变绒毛结构。
来自猪肠的肠激酶已用于一项研究中，以报告异常胰胆连接的新实验模型。来自猪肠的肠激酶也已用于研究胃抑制性多肽的促胰岛素区域的研究中。
生化/生理作用
肠激酶是一种膜结合的丝氨酸蛋白酶，可特异性和快速地将胰蛋白酶原转化为胰蛋白酶，从而触发其他酶原向活性酶的转化。它的分子量约为150 kDa。该酶是由35-47 kDa亚基组成的异二聚体。轻链和重链通过两个二硫键连接。它是一种含有35%碳水化合物的糖蛋白。胰蛋白酶原的多肽链仅在-（Asp）4-Lys-序列后水解。该酶被大豆胰蛋白酶抑制剂抑制。肠激酶通常用于蛋白修饰和氨基酸序列测定。
单位定义
在pH 5.6，25℃条件下，一个单位每分钟将从胰蛋白酶原产生1.0纳摩尔的胰蛋白酶。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
T Benhidjeb et al.
Journal of pediatric surgery, 31(12), 1670-1674 (1996-12-01)
A model of anomalous pancreatico-biliary junction was developed and used to investigate a possible role in the development of choledochal cyst and tumors of the biliary tract. An anastomosis was constructed between an isolated pancreas-duodenal segment and the gallbladder in
Werner Hartwig et al.
Surgery, 144(3), 394-403 (2008-08-19)
A noninvasive model of necrohemorrhagic pancreatitis induced by simultaneous intravenous cerulein/enterokinase (EK) infusion has recently been established in rats. The aim of the present study was to establish this new model in mice and to compare it with the rat
Complementary DNA cloning and sequencing of rat enteropeptidase and tissue distribution of its mRNA.
N Yahagi et al.
Biochemical and biophysical research communications, 219(3), 806-812 (1996-02-27)
A cDNA clone encoding enteropeptidase (EC 3.4.21.9), a key enzyme for the conversion of trypsinogen to trypsin, was isolated from a rat duodenal mucosa cDNA library. Sequences of the 3585 base pair clone predicted that enteropeptidase is synthesized as a
Thomas Thymann et al.
The British journal of nutrition, 97(6), 1128-1137 (2007-03-27)
The immediate post-weaning period is often associated with gut malfunction and diarrhoea for young pigs. Administration of antimicrobials remains an effective way to control weaning diarrhoea but it remains unclear how they affect gut physiology and microbiology although this is
Sara D F Santana et al.
Journal of biotechnology, 161(3), 378-382 (2012-07-24)
Magnetic nanobiocatalysts for tag cleavage on fusion proteins have been prepared by immobilizing enterokinase (EK) onto iron oxide magnetic nanoparticles coated with biopolymers. Two different chemistries have been explored for the covalent coupling of EK, namely carbodiimide (EDC coupling) and
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