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Merck
CN

D5030

杜氏改良 Eagle 培养基-低葡萄糖

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207

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产品名称

杜氏改良 Eagle 培养基-低葡萄糖, Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

Application

杜氏改良Eagle′培养基已用于：
  • 培养分离的指短屈肌（FDB）纤维，以测量外源脂肪酸（FA）的利用率，作为耗氧率（OCR）测量的一部分
  • 培养离体胫骨前肌纤维以测定乳酸含量
  • 培养用于成骨分化的人骨髓间充质干细胞，制备培养人肝细胞癌HepG2细胞的低糖培养基

General description

最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员，可以根据应用灵活优化配方。
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Preparation Note

按照每升培养基8.3克粉末配制。
补充1.0g/L葡萄糖，0.584g/L L-谷氨酰胺，3.7g/L碳酸氢钠。

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000451565
0000451565
0000447511
0000447512
0000447511

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