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D5030

Sigma-Aldrich

杜氏改良 Eagle 培养基-低葡萄糖

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

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别名:
DME, DMEM
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

500

形式

powder

技术

cell culture | mammalian: suitable

组分

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

相关类别

一般描述

最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员，可以根据应用灵活优化配方。
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。

应用

杜氏改良Eagle′培养基已用于：
  • 培养分离的指短屈肌（FDB）纤维，以测量外源脂肪酸（FA）的利用率，作为耗氧率（OCR）测量的一部分
  • 培养离体胫骨前肌纤维以测定乳酸含量
  • 培养用于成骨分化的人骨髓间充质干细胞，制备培养人肝细胞癌HepG2细胞的低糖培养基

数量

按照每升培养基8.3克粉末配制。

重悬

补充1.0g/L葡萄糖，0.584g/L L-谷氨酰胺，3.7g/L碳酸氢钠。

其他说明

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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GHS07

警示用语：

Warning

危险声明

H319

危险分类

Eye Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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