D0550
3,5-二硝基水杨酸
used in colorimetric determination of reducing sugars
别名:
2-羟基-3,5-二硝基苯甲酸, DNS
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关于此项目
线性分子式:
(O2N)2C6H2-2-(OH)CO2H
化学文摘社编号:
分子量:
228.12
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
210-204-3
Beilstein/REAXYS Number:
2220661
MDL number:
Assay:
≥98% (HPLC and titration)
Form:
powder
Quality Level
assay
≥98% (HPLC and titration)
form
powder
mp
168-172 °C (lit.)
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
InChI key
LWFUFLREGJMOIZ-UHFFFAOYSA-N
Application
已经使用 3，5-二硝基水杨酸（DNS）来停止体外 α-淀粉酶抑制研究中的反应。它还用于酶促释放的还原糖的定量。
Biochem/physiol Actions
3，5-二硝基水杨酸（DNS）用于还原糖的比色测定，并通过定量酶释放的还原糖来分析糖苷酶（糖苷水解酶）活性。已经将二硝基水杨酸方法与 Nelson-Somogi 比色法进行了比较。
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1
存储类别
4.1A - Other explosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
实验方案
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.
Mokni-Tlili S, et al.
World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)
Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
Gusakov, A.V., et al.
International Journal of Antimicrobial Agents, e283658-e283658 (2011)
Comparison of the 3,5-dinitrosalicylicacid and Nelson-Somogyi methods of assaying for reducing sugars and determining cellulase activity.
Breuil, C. and Saddler, J.N.
Enzyme and Microbial Technology, 7, 327-332 (1985)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D0550-500G | 04061835347377 |
| D0550-100G | 04061835347346 |
| D0550-25G | 04061835347360 |
| D0550-10G | 04061835347353 |