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D0426

Sigma-Aldrich

DAB with Metal Enhancer

SIGMAFAST™, peroxidase substrate, tablet

别名:

3,3′-二氨基联苯胺过氧化物酶底物, DAB过氧化物酶底物

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About This Item

UNSPSC代码:
12352204
NACRES:
NA.83

产品名称

SIGMAFAST™ DAB，含金属增强剂, tablet

表单

tablet

质量水平

200

溶解性

water: 1 tablet/5 mL

储存温度

−20°C

相关类别

一般描述

使用含金属强化剂的SIGMA FAST™ DAB可制备用于检测过氧化物酶活性的活性底物溶液，无需额外的缓冲液或步骤。常用作免疫组织学的沉淀底物。

应用

含金属强化剂的SIGMAFAST™ DAB 已被用于通过免疫组织化学观察肺组织切片、背根神经节（DRG）神经元和前十字韧带（ACL）细胞中胶原蛋白的免疫组织化学染色。
通过添加CoCl2可以增强DAB反应，并产生更强的耐酒精色斑。

重悬

将每个片剂组合溶于5 mL去离子水中，得到含有DAB和金属强化剂的即用型缓冲溶液。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

警示用语：

Danger

危险分类

Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

闪点(°F)

Not applicable

闪点(°C)

Not applicable

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCD4801
SLBP7387V
SLBJ7196V
SLBF4765V
061M8207V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?

    The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.

  4. What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?

    This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.

  5. How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?

    Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Saba Shahin et al.
Toxicological sciences : an official journal of the Society of Toxicology, 161(2), 349-374 (2017-10-27)
Microwave (MW) radiation induced oxidative stress reduces dendritic arborization, spine density and number of hippocampal pyramidal neurons and hence, impair learning and spatial memory through p53-dependent/independent apoptosis of hippocampal neuronal and nonneuronal cells. However, the mechanisms responsible for MW radiation
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 175, 254-268 (2017-09-20)
Birds time their daily and seasonal activities in synchronization with circadian and annual periodicities in the environment, which is mainly provided by changes in photoperiod/day length conditions. Photoperiod appears to act at the level of eye, pineal and encephalic/deep brain
M Breijo et al.
Journal of economic entomology, 110(3), 1390-1393 (2017-04-08)
The horn fly, Haematobia irritans (L.), is a blood-sucking livestock ectoparasite responsible for substantial livestock losses. In the present work, the potential use of recombinant hematobin (HTB), a horn fly salivary protein, as an antigen for cattle vaccination was investigated.
Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng M, et al.
Tissue Engineering: Part A, 16(5), 1479-1489 (2010)
Somanshu Banerjee et al.
Journal of photochemistry and photobiology. B, Biology, 176, 124-135 (2017-10-11)
In most of the avian species, daylength or photoperiod is the main environmental factor regulating reproduction. During their annual gonadal cycle, birds once sensitive to short or long day effect develop refractoriness to the same daylength and gonad develop or
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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

实验方案

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像，完成整个免疫组织化学（IHC）操作步骤。

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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