CS0760
线粒体分离物染色试剂盒
1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)
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关于此项目
UNSPSC Code:
12352207
EC Number:
217-896-6
NACRES:
NA.32
Technique(s):
flow cytometry: suitable, protein staining: suitable
Usage:
kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
Storage temp.:
−20°C
Detection method:
fluorometric
Shipped in:
dry ice
Quality Segment
usage
kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
flow cytometry: suitable, protein staining: suitable
fluorescence
λex 490 nm; λem 590 nm (JC-1 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
Application
独立的线粒体染色试剂盒已被用于评估线粒体内膜的电位。
Biochem/physiol Actions
本试剂盒基于JC-1染料的线粒体染色法。在正常细胞中，JC-1染料能够聚集于线粒体基质中，形成红色的荧光聚集物。任何导致线粒体膜电位消失的事件（如细胞凋亡）都会导致JC-1染料在线粒体内无法聚集。可通过荧光计或荧光显微镜来检测这一染料。
Features and Benefits
- 简单快捷的方法，可用于对线粒体分离产物进行染色和检测线粒体完整性
- 包含检测线粒体内膜电化学电位改变所需的全部试剂
- 包含能够穿透线粒体膜的抗菌性缬氨霉素，因此能够消除线粒体的电化学电位。缬氨霉素可用做对照试剂，来避免JC-1的聚集。
Analysis Note
本试剂盒用于检测线粒体的完整性，以及线粒体膜电位的消失事件。
仅试剂盒组分
产品编号
说明
- DMSO 1 mL
- JC-1 Assay Buffer 5X 25 mL
- JC-1 Stain 25 μg
- Valinomycin Ready Made 100 μL
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3