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主要文件

安全信息

CMC0016

Sigma-Aldrich

BL21(DE3)电感受态细胞

Escherichia coli, rod shaped

别名:

BL21 strain

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About This Item

UNSPSC代码:
41106202
NACRES:
NA.85

¥2,468.27

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产品名称

BL21(DE3)电感受态细胞, for protein expression

生物来源

Escherichia coli

等级

Molecular Biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

运输

dry ice

储存温度

−70°C

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application(s)

environmental
food and beverages
industrial qc
pharmaceutical

application(s)

environmental
food and beverages
industrial qc
pharmaceutical

application(s)

environmental
food and beverages
industrial qc
pharmaceutical
sample preparation

application(s)

cleaning products
cosmetics
environmental
flavors and fragrances
food and beverages
personal care

assay

≥99.5% (GC)

assay

-

assay

≥99.5% (GC)

assay

≥99.9% (GC)

Quality Level

200

Quality Level

-

Quality Level

300

Quality Level

200

grade

for analytical purposes, for extraction, for synthesis, for cleaning, for preparative purposes

grade

for analytical purposes, for cleaning, for extraction, for preparative purposes, for synthesis

grade

ACS reagent, for analytical purposes, for extraction, reagent grade

grade

analytical standard

solubility

85.3 g/L

solubility

-

solubility

85.3 g/L

solubility

water: soluble

form

liquid

form

liquid

form

liquid

form

-

一般描述

BL21（DE3）电转感受态细胞是第一个在相同细胞中实现高效克隆和高水平蛋白质表达的细胞。
克隆效率比其它 BL21 细胞制剂提高了 25-1,000 倍，这对于构建复杂的表达文库至关重要。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

特点和优势

BL21（DE3）电转感受态细胞（>5 × 109 cfu/μg）前所未有的转化效率消除了将质粒从克隆菌株转移到表达菌株的需要，从而为典型的克隆和表达实验节省了数天的工作时间。

组分

  • BL21（DE3）电转感受态细胞
  • pUC19转化对照DNA
  • 表达恢复培养基


储存分类代码

10 - Combustible liquids

法规信息

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  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    SLCM4783
    SLCP1550
    SLCM4784
    SLCJ4196
    SLCJ5997

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    Zev A Ripstein et al.
    eLife, 9 (2020-01-10)
    The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
    Julianne M Troiano et al.
    eLife, 10 (2021-01-16)
    Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
    Felix Nicolaus et al.
    eLife, 10 (2021-02-09)
    We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
    Brandon R Lowe et al.
    eLife, 10 (2021-02-02)
    Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation
    Salvatore Di Girolamo et al.
    Microbial cell factories, 19(1), 170-170 (2020-08-29)
    Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
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    实验方案

    BL21(DE3) Electrocompetent Cells

    Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

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