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主要文件

安全信息

CMC0012

Sigma-Aldrich

XLDNA SIG10 Electrocompetent cells

for BAC & cosmid cloning

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

生物来源

Escherichia coli

等级

Molecular Biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg

运输

dry ice

储存温度

−70°C

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此商品
R1780R7513R1145
technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

liquid

form

liquid

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

400

一般描述

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

特点和优势

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA

组分

  • XLDNA SIG10 electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


储存分类代码

10 - Combustible liquids

法规信息

高风险级别生物产品--病毒,疫苗等
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

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