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NIH 3T3 Cell Line murine

93061524, mouse embryo, Fibroblast

别名:

3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells

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About This Item

UNSPSC代码:
41106514

product name

NIH 3T3 Cell Line murine, 93061524

生物来源

mouse embryo

描述

Embryonic fibroblast

生长模式

Adherent

核型

Not specified

形态学

Fibroblast

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

相关类别

细胞系来源

Mouse Swiss NIH embryo

细胞系描述

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

应用

DNA transfection studies
NIH 3T3 Cell Line has been used to:
  • study calcium-mediated actin reset (CaAR) in response to physiological changes
  • study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
  • determine the viral titer of the retroviral construct -ST40hTNFα

培养基

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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