product name
A431 Cell Line human, squamous carcinoma, 85090402
生物来源
human skin
生长模式
Adherent
核型
Hypertriploid
形态学
Epithelial
产品
Not specified
受体
Epidermal growth factor (EGF)
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
细胞系描述
应用
- to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
- to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
- to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants
培养基
传代培养常规
其他说明
相关产品
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
How do I handle Cell Line frozen cells upon arrival?
Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
How do I resuscitate/thaw frozen Cell Line cells?
A protocol for thawing cells can be found in the ECACC handbook.
What medium should I use when I thaw the Cell Line cells?
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
What passage are my Cell Line cells?
If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
How many Cell Line cells are in the vial?
Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
Are my Cell Line cells mycoplasma tested?
ECACC routinely tests all manufactured cell banks for mycoplasma.
How to freeze down Cell Line cells to make a cell stock?
A protocol for freezing down cells for cell stocks can be found in the ECACC manual.
Are the Cell Line cells tested for viral pathogens?
HPA Cultures does not perform any viral testing on the cell lines.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.
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