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Merck
CN

C9017

Sigma-Aldrich

伴刀豆球蛋白A 来源于洋刀豆 (刀豆)

Sepharose conjugate, buffered aqueous suspension

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别名:
ConA
UNSPSC代码:
12161501
NACRES:
NA.32

生物来源

Canavalia ensiformis

质量水平

偶联物

Sepharose conjugate

形式

buffered aqueous suspension

包含

20% ethanol as preservative

标记范围

10-16 mg per mL

基质

Sepharose 4B

基质活化

cyanogen bromide

基质附着

amino

基质隔离区

1 atom

容量

≥25 mg/mL binding capacity (thyroglobulin)

储存温度

2-8°C

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应用

用于纯化含甘露糖的糖蛋白。

生化/生理作用

刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。

外形

悬浮于0.1 M醋酸盐缓冲液中,pH 6.0,含有1 M NaCl以及 CaCl2、MgCl2和MnCl2各1 mM

分析说明

在报道中,凝集活性以μg/ml表示,并且是在含有1mg/mL Ca 2+和Mn 2+的磷酸盐缓冲盐水(pH 6.8)的系列稀释液中进行测定的。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。

法律信息

Sepharose is a trademark of Cytiva

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

102.2 °F

闪点(°C)

39 °C

法规信息

动植物源性产品

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