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product name
胶原蛋白 来源于小牛皮, Bornstein and Traub Type I, (0.1% solution in 0.1 M acetic acid), aseptically processed, BioReagent, suitable for cell culture
生物来源
bovine (calf) skin
质量水平
无菌性
aseptically processed
产品线
BioReagent
形式
solution (0.1% solution in 0.1 M acetic acid)
包装
pkg of 20 mL
存货情况
not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)
浓度
(0.1% solution in 0.1 M acetic acid)
技术
cell culture | mammalian: suitable
表面覆盖度
6‑10 μg/cm2
结合特异性
Peptide Source: Collagen
Peptide Source: Elastin
Peptide Source: Fibronectin
运输
wet ice
储存温度
2-8°C
基因信息
bovine ... COL1A1(282187) , COL2A1(407142)
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相关类别
一般描述
应用
- 免疫荧光研究中载玻片的预先铺制
- 在神经元培养中，作为细胞粘附因子和聚偏二氟乙烯-三氟乙烯（P（VDF-TrFE））膜的修饰
- 在鼠胚成纤维细胞培养中用于培养皿的涂层
生化/生理作用
组分
制备说明
其他说明
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
How can I sterilize collagen solutions?
We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of collagen solution. DO NOT SHAKE OR STIR. Allow to stand overnight at 2-8 °C. Aseptically remove the top layer containing the collagen solution to a new bottle. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Can Product C8919, Collagen solution, be used to make 3-D collagen gels?
This product is NOT intended to produce 3-D (thick) gels, since the solution will not gel. This may be due to gamma-irradiation of the powder during the production process. We recommend using Product No C4243, which is tested and found to be suitable for 3-D gel formation.
Why does Product C8919, Collagen solution, look milky or contain particulates?
C8919, Collagen Solution from calf skin, is prepared by allowing irradiated sterile collagen strands to mix with sterile filtered 0.1N acetic acid over several hours. The collagen dissolves to an opalescent solution that is aseptically dispensed while stirring. No further filtration is necessary. Due to cross-linkage and sequence variability, as well as structural changes that can occur during isolation and purification, Collagen Type I (Bornstein and Traub) is a heterogeneous mixture rather than a pure compound. This variability, along with slight differences in the raw material, lends itself to shifts in the opacity of the solution from lot to lot. This product is tested for sterility prior to release for sale to our customers. A milky or particulate appearance is not indicative of microbial contamination and does not affect product use. .
How can I use Product C8919, Collagen solution, to coat my cultureware?
A protocol for coating cultureware can be found on the product information sheet (under Documents, above).
How long can plates coated with Product C8919, Collagen solution, be stored?
We make no recommendations about storage of plates after coating. Since factors such as humidity may impact on longevity of coated plates, customers should make their own determination as to whether plates can be stored and under which conditions. We used to offer SigmaScreen collagen coated plates as Product No. S3315. For optimal performance of that product, we recommend that the unopened product should be stored in a dry place at 2-8°C. We found that the S3315 could be stored at room temperature for up to three months, and that it should never be exposed to temperatures above 50°C.
Product C8919, Collagen solution, gelled while refrigerated. Can it still be used?
This can occur but does not harm the product. When warmed to room temperature, the product will liquify. Please make sure it is well mixed prior to dilution for coating cultureware.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
细胞外基质（ECM）是由细胞分泌并在组织中围绕在其周围。
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
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