C8176
胶原酶 来源于溶组织梭菌
Sigma Blend Type L, ≤1.0 FALGPA units/mg solid
别名:
梭菌肽酶 A
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≤1.0 FALGPA units/mg solid
Quality Level
form
powder
caseinase activity
≥40 units/mg solid
specific activity
≤1.0 FALGPA units/mg solid
mol wt
68-130 kDa
storage temp.
−20°C
Application
来自溶组织梭菌的胶原酶或梭菌肽酶A已被用于研究基于苯二胺芥的大分子抗肿瘤衍生物的合成和体外评价。梭菌肽酶A也已用于研究胶原酶对烧伤伤口的酶促清创术作用。
Biochem/physiol Actions
从组织中进行细胞的有效释放需要胶原酶和中性蛋白酶的作用。胶原酶被每克摩尔酶 4 克原子钙（Ca2+）活化。培养滤液被认为含有至少7种不同蛋白酶，分子量范围为68-130 kDa。最适pH为6.3-6.8。该酶通常用于消化组织样品中的结缔组分以释放单个细胞。已知乙二醇-双（β-氨基乙基醚）-N，N，N′，N′-四乙酸（EGTA）4；β-巯基乙醇；还原型谷胱甘肽；巯基乙酸，钠；和2，2′-联吡啶基；8-羟基喹啉抑制酶活性。
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
Other Notes
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
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实验方案
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
Coşkun Ozcan et al.
Burns : journal of the International Society for Burn Injuries, 28(8), 791-794 (2002-12-05)
Seventy-eight pediatric burn patients treated by enzymatic debridement with collagenase clostridiopeptidase A (CCA), were compared to 41 patients those burn wounds were excised surgically. Patients whose burn wounds were initially assessed as partial-thickness at admission were enrolled in the study.
Ruth M Williams et al.
STAR protocols, 2(2), 100414-100414 (2021-04-20)
In order to process samples by fluorescence-activated cell sorting (FACS), it is essential to obtain a single-cell suspension of dissociated cells. Numerous protocols and commercial reagents are available; however, each requires optimization for specific tissue types. Here, we describe an
Katleen De Winne et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 24(2-3), 159-168 (2005-01-22)
Poly-[N-(2-hydroxyethyl)-L-glutamine] (PHEG) and poly(ethylene glycol) (PEG)-grafted PHEG conjugates of N,N-di(2-chloroethyl)-4-phenylenediamine mustard (PDM) were synthetised. A collagenase-sensitive oligopeptide spacer was selected to link the cytotoxic agent PDM onto the polymeric carrier. First, the oligopeptide-drug conjugate, L-pro-L-leu-gly-L-pro-gly-PDM, was prepared. In a second
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C8176-1G | 04061826682203 |
| C8176-500MG | 04061832700304 |
| C8176-100MG | 04061832700281 |
| C8176-5G | 04061831157635 |
| C8176-25MG | 04061832700298 |