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安全信息

C7762

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

Type I-S, essentially salt-free, lyophilized powder

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别名:
α-chymotrypsin A and B, alpha-chymotrypsin
CAS号:
9004-07-3
EC 号:
3.4.21.1 (BRENDA, IUBMB)
EC 号:
232-671-2
MDL编号:
MFCD00130481
UNSPSC代码:
12352204
eCl@ss:
42010112
NACRES:
NA.54

类型

Type I-S

质量水平

200

形式

essentially salt-free, lyophilized powder

比活

≥40 units/mg protein

分子量

25 kDa

纯化方式

3× crystallization

溶解性

1 mM HCl: soluble 2.0 mg/mL, clear

UniProt登记号

P00767

储存温度

−20°C

基因信息

cow ... CTRB1(618826)

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相关类别

应用

在一项研究中使用了来自牛胰腺的 α-糜蛋白酶，以研究通过 Winsor-III 微乳液系统提取蛋白质。来自牛胰腺的 α-胰凝乳蛋白酶也被用于研究胰蛋白酶的一种新的基于富勒烯的特异性荧光探针。
该产品已被用于研究几种醚低聚物对酶的抑制作用。它也被用于裂解酚氧化酶前体，顺序估算血淋巴中的总酚氧化酶。此外，在使用 Deraeocoris nigritulus 的唾液腺和前中肠提取物的糜蛋白酶试验中，将该酶用作阳性对照。

生化/生理作用

α-糜蛋白酶是一种丝氨酸肽酶，在由二硫键连接的三条多肽链（a 链-13 残基、B 链-131 残基和 C 链-97 残基）中含有 241 个氨基酸残基。发现该酶的分子量为 25 kDa。其 pI 为 8.75。选择性水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸 C 末端的肽键。ca2+ 激活并稳定酶。该酶被二异丙基氟磷酸盐 (DFP)、苯甲基磺酰氟 (PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮 (TPCK)、糜蛋白酶抑制剂、抑肽酶、α10-抗胰蛋白酶、α2-巨球蛋白、10 mM cu2 + 和 hg2 + 抑制。
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

单位定义

在 pH 7.8、25°C 条件下，1 个单位将每分钟水解 1.0 μmol BTEE。

制备说明

制备无自溶产物和低分子量污染物。
可在 1 mM HCl 中复溶 2 mg/mL 浓度的粉末，以形成澄清溶液。

分析说明

E1%/280法测定蛋白
最低 85% 蛋白质

其他说明

有关胰凝乳蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

底物

产品编号
说明
价格

抑制剂

产品编号
说明
价格

A2504

6-氨基己酸, ≥99% (titration), powder

A7824

6-氨基己酸, BioUltra, ≥99%

D0879

氟磷酸二异丙酯

L2023

亮肽素 三氟乙酸盐, ≥90% (HPLC), microbial

T9777

胰蛋白酶-胰凝乳蛋白酶抑制剂 来源于大豆, lyophilized powder

A1153

抑肽酶 来源于牛肺, lyophilized powder, 3-8 TIU/mg solid

P7626

苯甲磺酰氟, ≥98.5% (GC)

B1552

溴烯醇内酯, ≥98% (TLC)

A9024

α1-抗胰蛋白酶 来源于人类血浆, salt-free, lyophilized powder

A6150

α1-抗胰蛋白酶 来源于人类血浆, salt-free, lyophilized powder

A9285

α1-Antichymotrypsin 来源于人类血浆, ≥92.5% (SDS-PAGE), lyophilized powder

T4376

N-对-甲苯磺酰基-L-苯基乙基氯甲基酮, ≥97% (TLC), powder

C7268

胰凝乳蛋白酶抑制剂, microbial

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How should I prepare a solution of α-Chymotrypsin, Product C7762?

    It is recommended to reconstitute product C7762 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme.

  6. How can solutions of  α-Chymotrypsin, Product C7762, be stored?

    Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride can be stored at -20 °C for about one week.

  7. What is the pH optimum for the enzyme, α-Chymotrypsin, Product C7762?

    The pH optimum is between pH 7.5-8.5.

  8. What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C7762)?

    The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.  This information and the basic structure description of the enzyme can be found on our product information sheet (under Documents, above).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Olivier Renaudet et al.
Organic letters, 6(3), 397-400 (2004-01-30)
[structure: see text] Hydroxyaromatic aldehydes and ketones were used as building blocks to prepare ether oligomers. An iterative two-step protocol involving Mitsunobu coupling and carbonyl reduction provided a protecting-group-free route with high yields. Activity screening of an 84-member library against
Nathan W Bailey et al.
Biology letters, 7(2), 217-220 (2010-09-03)
Increased investment in immunity is expected to be beneficial under crowded conditions because of the greater risk of pathogen and parasite transmission, but the evolution of this facultative response relies on the ability to accurately assess social cues in the
Digestive enzymes and stylet morphology of Deraeocoris nigritulus (Uhler)(Hemiptera: Miridae) reflect adaptations for predatory habits.
Boyd Jr, D. W.
Annals of the Entomological Society of America, 96(5), 667-671 (2003)
Lakshmi Swarnalatha Jasti et al.
Biotechnology progress, 30(2), 317-323 (2014-01-23)
Allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer with 25% crosslink density (AGE-25) shows excellent bovine serum albumin (BSA) adsorption (up to 16% (w/w)) at pH 8.0 and the adsorbed BSA is strongly bound. This protein-coated polymer provides a novel
J A Hodgson et al.
Journal of general microbiology, 131(12), 3219-3227 (1985-12-01)
The effects of elevated temperature and of digestion with a variety of proteinases on the flocforming ability of flocculent strains of Saccharomyces cerevisiae, both genetically defined (FLO1 and FLO5) laboratory and genetically undefined brewing strains, have been determined. This has
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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

α-胰凝乳蛋白酶的酶学测定程序（EC 3.4.21.1）

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

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