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Merck
CN

C6423

α-Chymotrysin Protease

Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas

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化学文摘社编号:
9004-07-3
UNSPSC Code:
12352204
eCl@ss:
42010112
NACRES:
NA.26
EC Number:
232-671-2
MDL number:
MFCD00130481
EC 号:
3.4.21.1 (BRENDA, IUBMB)

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产品名称

α-糜蛋白酶 来源于牛胰腺, suitable for protein sequencing, salt-free, lyophilized powder

grade

Proteomics Grade

form

salt-free, lyophilized powder

mol wt

25 kDa

suitability

suitable for protein sequencing

UniProt accession no.

P00767

storage temp.

2-8°C

Quality Level

200

Gene Information

cow ... CTRB1(618826)

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Other Notes

在 pH 7.8、25°C 条件下，1 个单位将每分钟水解 1.0 μmol BTEE。
查看更多信息 糜蛋白酶 的信息，请访问 www.sigma-aldrich.com/enzymeexplorer

Application

牛胰 α-糜蛋白酶有以下用途：
  • 通过质谱法 (MS) 鉴定蛋白质
  • 肌球蛋白重链的分离与鉴定
  • 毒素制剂
  • 用于 SDS-PAGE 分析细胞蛋白的感染和未感染细胞孵育
牛胰 α-糜蛋白酶 (BPC) 可催化 Povarov 反应制备四氢喹啉衍生物。

Biochem/physiol Actions

α-牛胰腺胰凝乳蛋白酶通过Ca2+实现稳定并且催化肽键的水解，尤其氨基酸酪氨酸、苯基丙氨酸、色氨酸以及C端亮氨酸。α-胰凝乳蛋白酶受Cu2+和Hg的抑制。
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

General description

牛胰 α-糜蛋白酶 (BPC) 可催化 Povarov 反应制备四氢喹啉衍生物。牛胰 α-糜蛋白酶 (CHT) 是一种酶蛋白。已报道乙醇、1,4-二恶烷和乙腈等不同浓度的有机溶剂对 CHT 的影响。
胰凝乳蛋白酶(Chy)是一种丝氨酸蛋白酶。它的分子量为25.7kDa，广泛应用于医药行业。它由胰凝乳蛋白酶原合成，这种转化过程需要钙。

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

动植物源性产品
低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000479512
0000479512
0000424285
0000424285
0000375784

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L M Simon et al.
Biochemical and biophysical research communications, 280(5), 1367-1371 (2001-02-13)
The effects of different concentrations (20-95%) of organic solvents (ethanol, 1,4-dioxane and acetonitrile) were studied on alpha-chymotrypsin and trypsin from bovine pancreas. The changes in secondary structure were followed by CD measurements, and the apparent Michaelis constants (KMapp) and the
S Herrero et al.
Applied and environmental microbiology, 67(3), 1085-1089 (2001-03-07)
Susceptibility to protoxin and toxin forms of Cry1Ab and the binding of (125)I-labeled Cry1Ab and Cry1Ac has been examined in three Plodia interpunctella colonies, one susceptible (688(s)) and two resistant (198(r) and Dpl(r)) to Bacillus thuringiensis. Toxicological studies showed that
A new platform for chymotrypsin isolation from fresh bovine pancreas using an environmental friendly polyelectrolyte: Alginate
Rausch C, et al.
BioChemistry: An Indian Journal, 9(6), 213-220 (2015)
Tessa E F Quax et al.
Virology, 404(1), 1-4 (2010-05-22)
Recently a unique mechanism of virion release was discovered in Archaea, different from lysis and egress systems of bacterial and eukaryotic viruses. It involves formation of pyramidal structures on the host cell surface that rupture the S-layer and by opening
Xinyu Liu et al.
eLife, 9 (2020-12-05)
The production of reactive oxygen species (ROS) is a ubiquitous defense response in plants. Adapted pathogens evolved mechanisms to counteract the deleterious effects of host-derived ROS and promote infection. How plant pathogens regulate this elaborate response against ROS burst remains
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Product Information Sheet - C6423

Product Information Sheet

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