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等级
Proteomics Grade
质量水平
表单
salt-free, lyophilized powder
分子量
25 kDa
适用性
suitable for protein sequencing
UniProt登记号
储存温度
2-8°C
基因信息
cow ... CTRB1(618826)
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一般描述
牛胰 α-糜蛋白酶 (BPC) 可催化 Povarov 反应制备四氢喹啉衍生物。牛胰 α-糜蛋白酶 (CHT) 是一种酶蛋白。已报道乙醇、1,4-二恶烷和乙腈等不同浓度的有机溶剂对 CHT 的影响。
胰凝乳蛋白酶(Chy)是一种丝氨酸蛋白酶。它的分子量为25.7kDa,广泛应用于医药行业。它由胰凝乳蛋白酶原合成,这种转化过程需要钙。
应用
牛胰 α-糜蛋白酶有以下用途:
- 通过质谱法 (MS) 鉴定蛋白质
- 肌球蛋白重链的分离与鉴定
- 毒素制剂
- 用于 SDS-PAGE 分析细胞蛋白的感染和未感染细胞孵育
生化/生理作用
α-牛胰腺胰凝乳蛋白酶通过Ca2+实现稳定并且催化肽键的水解,尤其氨基酸酪氨酸、苯基丙氨酸、色氨酸以及C端亮氨酸。α-胰凝乳蛋白酶受Cu2+和Hg的抑制。
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。
单位定义
在 pH 7.8、25°C 条件下,1 个单位将每分钟水解 1.0 μmol BTEE。
其他说明
查看更多信息 糜蛋白酶 的信息,请访问 www.sigma-aldrich.com/enzymeexplorer
底物
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价格
抑制剂
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警示用语:
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Xinyu Liu et al.
eLife, 9 (2020-12-05)
The production of reactive oxygen species (ROS) is a ubiquitous defense response in plants. Adapted pathogens evolved mechanisms to counteract the deleterious effects of host-derived ROS and promote infection. How plant pathogens regulate this elaborate response against ROS burst remains
Tessa E F Quax et al.
Virology, 404(1), 1-4 (2010-05-22)
Recently a unique mechanism of virion release was discovered in Archaea, different from lysis and egress systems of bacterial and eukaryotic viruses. It involves formation of pyramidal structures on the host cell surface that rupture the S-layer and by opening
Miguel Ribeiro et al.
International journal of molecular sciences, 14(3), 5650-5667 (2013-03-13)
Analysis of Portuguese wheat (Triticum aestivum L.) landrace 'Barbela' revealed the existence of a new x-type high molecular weight-glutenin subunit (HMW-GS) encoded at the Glu-A1 locus, which we named 1Ax1.1. Using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared
The α-chymotrypsin-catalyzed Povarov reaction: one-pot synthesis of tetrahydroquinoline derivatives.
Li LP, et al.
Green Chemistry, 17(5), 3148-3156 (2015)
Methods in molecular biology. v. 16
Burrell, MM
BioChemistry: An Indian Journal (1993)
商品
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
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