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Merck
CN

C6164

Sigma-Aldrich

细胞冷冻培养基-DMSO 1×

sterile-filtered, suitable for cell culture

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别名:
cryopreservation medium
UNSPSC代码:
12352207
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

形式

liquid

技术

cell culture | mammalian: suitable
cryopreservation: suitable

杂质

endotoxin, tested

运输

dry ice

储存温度

−20°C

一般描述

细胞培养中的细胞冷冻或超低温保存是常规过程。存储过程中保持细胞完整性至关重要。主要使用的冷冻保护剂包括甘油,乙二醇和二甲基亚砜(DMSO)。

应用

细胞冷冻培养基-DMSO 1x用于超低温冷冻保存人类胚胎肾脏 HEK293 CNR2细胞、血液单核细胞和前列腺癌细胞。

其他说明

完全即用型培养基。 含有最低必需培养基、二甲基亚砜、小牛血清和胎牛血清的专利配方。

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?

    The DMSO is at a 10% concentration.

  6. Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum? 

    No, the serum in this cell freezing media is not heat-inactivated.

  7. Is there insulin in Product C6164, Cell Freezing Medium-DMSO?

    No, there is no added insulin in this product.  There may be traces of insulin from the serum used in this product.

  8. Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?

    Product No. C6164 is sterile-filtered.  If the sterility of the product has been compromised, it can be re-filtered.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Safety and immunogenicity of oral microencapsulated enterotoxigenic Escherichia coli surface antigen 6 (CS6) with and without LTR192G: a randomized clinical trial
Lapa JA, et al.
Clinical and Vaccine Immunology : CVI (2008)
Variations of components of the plasminogen activation system with the cell cycle in benign prostate tissue and prostate cancer
Plas E, et al.
Cytometry, 46(3), 184-189 (2001)
Antonia Patsialou et al.
Cancer research, 69(24), 9498-9506 (2009-11-26)
Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased
Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Bahari L, et al.
Testing, 13(2), e0192265-e0192265 (2018)
Scott A Jackson et al.
BMC genomics, 12, 349-349 (2011-07-08)
The gene content of a diverse group of 183 unique Escherichia coli and Shigella isolates was determined using the Affymetrix GeneChip® E. coli Genome 2.0 Array, originally designed for transcriptome analysis, as a genotyping tool. The probe set design utilized

商品

用于细胞系冷冻保存的良好细胞库操作实践概述。

Overview of good cell banking practices for cell line cryopreservation purposes.

实验方案

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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