生物来源
bacterial (Clostridium histolyticum)
质量水平
形式
solid
比活
≥800 CDU/mg solid
2-5 FALGPA units/mg solid
分子量
68-130 kDa
运输
dry ice
储存温度
−20°C
基因信息
rat ... LOC296948(296948)
应用
胶原酶与蛋白酶抑制剂已被用于一项评估免疫系统基因多态性与数量特征关系的研究。它也被用于研究 溶组织梭菌 胶原酶抑制剂的定量构效关系。sigma 酶已用于猪胰岛的分离。它也用于分离小鼠胰腺浸润的淋巴细胞。
生化/生理作用
有效地从组织中释放细胞需要胶原酶和中性蛋白酶的共同操作。每摩尔酶中 4 克钙 (Ca 2+) 可激活胶原酶。培养滤液被认为含有至少 7 种不同的蛋白酶，分子量范围为 68-130 kDa。最佳 pH 值为 6.3-8.8。这种酶通常用于消化组织样本中的连接成分，以释放单个细胞。胶原酶处理可使部分细胞死亡。通常使用 0.1 至 5 mg/mL 的浓度进行消化。反应持续时间可从 15 min 至数小时不等，以获得满意的细胞解离，而不会引起太多的细胞死亡。活性需要 Zn 2+。如果胶原酶不需要明显的蛋白酶活性，则使用本品。
其他说明
所选批次的胶原酶 XI 与蛋白酶抑制剂混合，以限制胰腺组织中的胰蛋白酶活性。该制剂是通过与多个外部实验室合作开发的。
质量
还含有梭菌蛋白酶和非特异性中性蛋白酶活性。
抑制剂
产品编号
说明
价格
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
Ashutosh Jamloki et al.
Bioorganic & medicinal chemistry letters, 16(14), 3847-3854 (2006-05-10)
A quantitative structure-activity relationship (QSAR) study has been performed on 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases (MMPs) and a bacterial collagenase known as Clostridium histolyticum collagenase (ChC) to understand the structural features influencing the affinity of these inhibitors towards the
Association of immune system gene polymorphisms with quantitative features which are pathogenetically important in chronic viral hepatitis.
Goncharova, I., et al.
Molecular Biology, 42, 242-246 (2008)
Austin P Huffman et al.
JCI insight, 5(10) (2020-04-24)
The role CD4+ T cells play in tumor immunity is less well appreciated than the cytotoxic role of CD8+ T cells. Despite clear evidence for CD4+ T cell dependency across multiple immunotherapies, the mechanisms by which CD4+ T cells infiltrate
C J Swanson et al.
Human immunology, 62(7), 739-749 (2001-06-26)
Recent progress in human islet transplantation demonstrates the feasibility of using purified human islets for treatment of type 1 diabetes mellitus; however, a shortage of human pancreata remains a major obstacle. This report describes methods to isolate porcine islets using
Brandon M Blobner et al.
American journal of physiology. Gastrointestinal and liver physiology, 321(5), G449-G460 (2021-09-16)
Chronic pancreatitis (CP) is a complex inflammatory disorder with numerous associated genetic and environmental risk factors. The most distressing characteristic of CP is recalcitrant pain, often requiring surgical resection including total pancreatectomy with islet autotransplantation (TPIAT). We studied five consented
实验方案
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门