C4982
细胞色素P450 人
3A4 isozyme microsomes, with P450 reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
别名:
P450酶, 人细胞色素P450, 细胞色素P450酶
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关于此项目
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
Concentration:
≥2 mg/mL (Bichinchonic acid method)
产品名称
细胞色素P450 人, 3A4 isozyme microsomes, with P450 reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
biological source
human
recombinant
expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
form
solution
mol wt
45-60 kDa
packaging
vial of ~0.5 nmol
vial of ≥25 units
concentration
≥2 mg/mL (Bichinchonic acid method)
technique(s)
co-immunoprecipitation (co-IP): suitable
solubility
water: soluble
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... CYP3A4(1576)
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Application
细胞色素P450已用于 体外结合试验，用于理解通过 haem–haem堆叠进行的蛋白质二聚化及其在癌症中的重要性。它也用于研究PGRMC1 和CYP3A4的体外结合。
Biochem/physiol Actions
细胞色素P450是异质性同工酶家族，其主要功能是氧化小分子，发挥中间代谢（如脂肪酸）功能和对外源性化合物（药物或毒素）进行解毒。有些同工型具有有限的底物特异性，而其他同工酶则比较混杂。CYP1A1亚型催化乙氧基试卤灵的7-脱乙基。细胞色素P450(CYP)在外源物解毒、细胞代谢和稳态中起重要作用。药物相互作用的一种主要机制就是激活或抑制这类酶。CYP酶可被多种外源物和内源底物经由受体依赖途径转录激活。基于代谢的药物相互作用主要影响就是抑制这类酶，许多化疗药物可通过抑制或诱导细胞色素P450酶系引发药物互作。
细胞色素P450（CYP）酶与许多反应有关，包括O-去烷基化、环氧化、S-氧化和羟基化。
General description
研究领域：IMMUNO和 CKS
细胞色素P450（CYP450）是一种膜结合血红素蛋白，这种血红素蛋白形成了一个参与外源和内源化合物代谢的庞大亚铁血红素-硫醇盐(heme-thiolate)蛋白超家族。这些 CYP450酶具有一个活性亚铁血红素铁中心，该铁中心通过高度保守的半胱氨酸硫醇盐配体来结合蛋白质分子。
细胞色素P450（CYP450）是一种膜结合血红素蛋白，这种血红素蛋白形成了一个参与外源和内源化合物代谢的庞大亚铁血红素-硫醇盐(heme-thiolate)蛋白超家族。这些 CYP450酶具有一个活性亚铁血红素铁中心，该铁中心通过高度保守的半胱氨酸硫醇盐配体来结合蛋白质分子。
Other Notes
含重组人CYP3A4和重组兔NADPH-P450还原酶的微粒体
在37℃，pH 7.4的条件下，一个单位每分钟将1纳摩尔甲状腺酮转化为6β-羟基酮。
Physical form
溶于 100 mM 磷酸钾缓冲液 (pH 7.4) 中。
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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Andreia Palmeira et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 15(1), 31-45 (2012-03-01)
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