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生物来源
human
质量水平
重组
expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
形式
solution
分子量
45-60 kDa
包装
vial of ~0.5 nmol
vial of ≥25 units
UniProt登记号
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
human ... CYP3A4(1576)
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一般描述
Research area: IMMUNO AND CKS
Cytochrome P450 (CYP450) is a membrane-bound hemoprotein that constitutes a vast superfamily of heme-thiolate proteins engaged in the metabolism of exogenous and endogenous compounds. These CYP450 enzymes feature an active heme iron center that is bound to a protein molecule via a highly conserved cysteine thiolate ligand.
Cytochrome P450 (CYP450) is a membrane-bound hemoprotein that constitutes a vast superfamily of heme-thiolate proteins engaged in the metabolism of exogenous and endogenous compounds. These CYP450 enzymes feature an active heme iron center that is bound to a protein molecule via a highly conserved cysteine thiolate ligand.
应用
Cytochrome P450 human has been used in in vitro binding assays to understand protein dimerization via haem–haem stacking and its significance in cancer. It has also been used to study in vitro binding between PGRMC1 and CYP3A4.
生化/生理作用
Cytochrome P450 (CYP) enzymes are associated with many reactions including O-dealkylation, epoxidation, S-oxidation, and hydroxylation.
细胞色素 P450 是一个具有异质性的同工酶家族，其主要功能是氧化小分子，既是中间代谢（如脂肪酸）的功能，也是对外源化合物（药物或毒素）解毒的功能。一些亚型具有较窄的底物特异性，而另一些则是替代的。
其他说明
含重组人CYP3A4和重组兔NADPH-P450还原酶的微粒体
单位定义
在37℃，pH 7.4的条件下，一个单位每分钟将1纳摩尔甲状腺酮转化为6β-羟基酮。
外形
溶于 100 mM 磷酸钾缓冲液 (pH 7.4) 中。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Andreia Palmeira et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 15(1), 31-45 (2012-03-01)
Aminated thioxanthones have recently been described as dual-acting agents: growth inhibitors of leukemia cell lines and P-glycoprotein (P-gp) inhibitors. To evaluate the selectivity profile of thioxanthones as inhibitors of multidrug resistance (MDR), their interaction with other ABC transporters, which were
Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance
Kabe Y, et al.
Nature Communications, 7, 11030-11030 (2016)
Cytochrome P450 in Cancer Susceptibility and Treatment
Mittal B, et al.
Advances in Clinical Chemistry, 71, 77-139 (2015)
Yasuaki Kabe et al.
Cancers, 13(13) (2021-07-03)
Progesterone receptor membrane component 1 (PGRMC1) is highly expressed in various cancer cells and contributes to tumor progression. We have previously shown that PGRMC1 forms a unique heme-stacking functional dimer to enhance EGF receptor (EGFR) activity required for cancer proliferation
Engineering Cytochrome P450 Biocatalysts for Biotechnology, Medicine, and Bioremediation
Kumar S
Expert Opinion on Drug Metabolism & Toxicology, 6(2), 115?131-115?131 (2010)
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