C4618
Monoclonal Anti-Cathepsin L antibody produced in mouse
clone CPL33/1, purified from hybridoma cell culture
别名:
Anti-CATL, Anti-CTSL, Anti-MEP
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About This Item
克隆:
CPL33/1, monoclonal
种属反应性:
human
技术:
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
CPL33/1, monoclonal
形式
buffered aqueous solution
分子量
antigen ~25 kDa (human cathepsin L)
antigen ~42 kDa (human pro cathepsin L)
种属反应性
human
浓度
~2 mg/mL
技术
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CTSL1(1514)
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一般描述
Anti-Cathepsin L antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human procathepsin L.
Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from BALB/c mice immunized with human procathepsin L.
特异性
The antibody recognizes the native and denaturated forms of the protein and does not cross react with human cathepsin V. Monoclonal Anti-Cathepsin L specifically recognizes human cathepsin L (∼ 25 kDa) and procathepsin L (∼ 42 kDa). Anti-Cathepsin L antibody epitope resides within amino acids of human cathepsin L (FYKE).
免疫原
human procathepsin L. The antibody epitope resides within amino acids 258-261 of human cathepsin L (FYKE).
应用
Anti-Cathepsin L antibody has been used for immunoblotting.
Monoclonal Anti-Cathepsin L antibody produced in mouse is suitable for:
- immunohistochemistry
- indirect ELISA
- western blot : 0.1-0.2 μg/mL using total cell extracts of A549 cells
生化/生理作用
Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis.
Inhibition of the enzyme or the proenzyme by low molecular weight inhibitors or by specific antibodies led to a suppression of the invasive capabilities of malignant cells, or a decline in their ability to form tumors in experimental in vivo and in vitro models.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
常规特殊物品
Cheuk-Yiu Law et al.
Biochemistry and biophysics reports, 5, 335-345 (2016-01-14)
Patients with Danon disease may suffer from severe cardiomyopathy, skeletal muscle dysfunction as well as varying degrees of mental retardation, in which the primary deficiency of lysosomal membrane-associated protein-2 (LAMP2) is considerably associated. Owing to the scarcity of human neurons
Cysteine cathepsins and the cutting edge of cancer invasion.
Gocheva V and Joyce J A
Cell Cycle, 6(1), 60-64 (2007)
E Weber et al.
Hybridoma, 16(2), 159-166 (1997-04-01)
Mouse monoclonal antibodies directed against cathepsin L and procathepsin L have been generated. Mice were immunized with human procathepsin L purified from the cell culture medium of human nonsmall cell lung cancer cell line EPLC 32 M1. More than 400
Boris Turk et al.
FEBS letters, 581(15), 2761-2767 (2007-06-05)
Proteases were, for a long time, mainly considered as protein degrading enzymes. However, in the last decade this view has changed dramatically, and the focus is now on proteases as signalling molecules. One of the best examples is apoptosis, the
Myung Jong Kim et al.
JCI insight, 9(3) (2024-02-08)
The glucocerebrosidase (GCase) encoded by the GBA1 gene hydrolyzes glucosylceramide (GluCer) to ceramide and glucose in lysosomes. Homozygous or compound heterozygous GBA1 mutations cause the lysosomal storage disease Gaucher disease (GD) due to severe loss of GCase activity. Loss-of-function variants
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