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Merck
CN
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文件

安全信息

C-41220

Sigma-Aldrich

DetachKit

3 x 250 ml

别名:

Cell Detachment Solution

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

包装

pkg of 3 × 250 ml

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−20°C

一般描述

DetachKit-250

应用

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

组分

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

质量

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

储存及稳定性

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

传代培养常规

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储存分类代码

10 - Combustible liquids

法规信息

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