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product name
I型胶原蛋白 溶液 来源于大鼠尾, BioReagent, suitable for cell culture, sterile-filtered
生物来源
rat tail
质量水平
描述
Approx. 100mg/vial ((35 - 40 ml) )
无菌性
sterile-filtered
产品线
BioReagent
检测方案
>95% (SDS-PAGE)
形式
liquid
分子量
apparent mol wt 115-130 kDa by SDS-PAGE (doublet)
apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
包装
pkg of 1 vial
浓度
>0.5 mg/mL Biuret
技术
cell culture | mammalian: suitable
表面覆盖度
6‑10 μg/cm2
杂质
Mycoplasma Test by Barile, none detected
溶解性
water: 1 mg/mL, clear to hazy, colorless (with 2 ul acetic acid or 0.1 N acetic acid)
NCBI登记号
UniProt登记号
结合特异性
Peptide Source: Collagen
Peptide Source: Fibronectin
运输
wet ice
储存温度
2-8°C
基因信息
rat ... Col1a1(29393)
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相关类别
一般描述
应用
生化/生理作用
特点和优势
- 来自大鼠尾。
- 通过除菌过滤尽可能降低污染风险。
- 适合广泛的细胞培养应用。
- 提供最适覆盖度(6-10 μg/cm2)，促进细胞黏附和增殖。
制备说明
SDS聚丙烯酰胺凝胶电泳分析显示I型胶原蛋白的典型条带模式是具有表观分子量为115和130kDa的双峰，以及215和235kDa的另一个双峰。 基于该分析可知胶原蛋白的纯度为>95%。
其他说明
相关产品
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
How are collagen solutions stored?
Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated.
Why am I seeing some insolubles in my collagen solution?
The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
How can I make a 3-D collagen gel?
Not all lots of C7661 are suitable for 3D gels. Product No. C4243 has been use-tested in this application. If C4243 is not available, we recommend that customers screen lots of C7661 to try to make 3D gels. We have found that collagen that has been sterilized by gamma-irradiation or by chloroform cannot be used to make 3D collagen gels. The following protocol can be used to make collagen gels: Materials required:Collagen gel solution 1.5 - 3 mg/mL 10X tissue culture medium containing phenol red Sodium bicarbonate or HEPES buffer Procedure: 1.Measure out 800 μL of collagen solution. 2.Add 100 μL of 10X medium (buffered with 1X sodium bicarbonate or HEPES) 3.Adjust pH with 1 N sodium hydroxide, if required (100 μl or less) 4.Add 10X medium to bring volume to 1 ml 5.Mix contents well. Solution should maintain red color to indicate physiological pH6. Dispense into wells to a depth of 1-2 mm (approx. 15 μL/well) 7.Transfer to 37 °C for 20-40 minutes 8.Examine for gel formation The above volumes represent quantities for use in 24 well plates. Volumes can be adjusted to accommodate any culture vessel.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
细胞外基质（ECM）是由细胞分泌并在组织中围绕在其周围。
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
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