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安全信息

C1184

Sigma-Aldrich

纤维素酶 来源于黑曲霉

greener alternative

powder, ≥0.3 units/mg solid

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别名:
1,4-(1,3:1,4)-β-D-葡聚糖4-葡聚糖水解酶
CAS号:
9012-54-8
EC 号:
3.2.1.4 (BRENDA, IUBMB)
EC 号:
232-734-4
MDL编号:
MFCD00081510
UNSPSC代码:
12352204
NACRES:
NA.54

形式

powder

质量水平

200

比活

≥0.3 units/mg solid

环保替代产品特性

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

, Enabling

储存温度

2-8°C

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相关类别

一般描述

如果生产过程中使用非致病性和非产毒菌株，同时利用目前良好的生产实践，FDA将从 A.niger 中获得的纤维素酶确认为GRAS（一般认为安全）。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品已用作替代能源的酶。欲了解更多，请参阅替代能源的酶研究。

纤维素酶属于糖苷水解酶类，由各种纤维素分解微生物分泌。

应用

来自Sigma的纤维素酶已被用于研究其几种可能的底物、纤维素、Avicel PH-101和滤纸，在动物和禽类消化道的单胃消化过程中保护酶活性的能力。
该酶还被批准作为辅助蛤虾加工的二级直接食品添加剂。

生化/生理作用

来自 黑曲霉（Aspergillus niger） 的纤维素酶可催化纤维素、地衣素、大麦葡聚糖和纤维低聚糖中的内切-1,4-β-D-糖苷键水解为纤维己糖。 其不会切割纤维二糖或 p-硝基苯基-β-D-葡萄糖苷。 该酶还可通过水解木糖基丝氨酸键从核心肽中切割完整的糖胺聚糖。

单位定义

在pH 5.0，37℃条件下，一个单位可在1小时内从纤维素中释放出1.0μmole的葡萄糖（孵育时间为2小时）。

其他说明

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer

底物

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solution stability of Cellulase, Product C1184?

    Sigma-Aldrich has not determined the solution stability for this product.  However, it is reported in Methods in Enzymology, 160, 264 (1988) that cellulase from Aspergillus niger is "completely stable over the range of pH 5.0-8.0 at 4°C for 24 hours and retains about 50% of its original activity after heating at 70°C for 10 minutes.  The enzyme is completely inactivated by heating at 80°C for 10 minutes".

  6. Are there any additives to Cellulase, Product C1184, in addition to the enzyme itself?

    Per information from our supplier, maltodextrin is an extender in this product.  Maltodextrin can vary in glucose chain lengths of 2-12. There can also be some free glucose in the product. Either sorbitol or propylene glycol is added to the product as a stabilizer during processing. These components may be "flashed off" during processing, but some small amount may still be present.

  7. What is the molecular weight of Product C1184, Cellulase from Aspergillus niger?

    The molecular weight is 26,000 Daltons.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Structural organization and a standardized nomenclature for plant endo-1, 4-beta-glucanases (cellulases) of glycosyl hydrolase family 9
Urbanowicz BR, et al.
Plant Physiology, 144(4), 1693-1696 (2007)
Miguel A Matilla et al.
Environmental microbiology, 13(7), 1745-1766 (2011-05-11)
GGDEF and EAL/HD-GYP protein domains are responsible for the synthesis and hydrolysis of the bacterial secondary messenger cyclic diguanylate (c-di-GMP) through their diguanylate cyclase and phosphodiesterase activities, respectively. Forty-three genes in Pseudomonas putida KT2440 are putatively involved in the turnover
E Schuster et al.
Applied microbiology and biotechnology, 59(4-5), 426-435 (2002-08-13)
Aspergillus niger is one of the most important microorganisms used in biotechnology. It has been in use already for many decades to produce extracellular (food) enzymes and citric acid. In fact, citric acid and many A. niger enzymes are considered
Fuxi Shi et al.
ACS omega, 5(38), 24780-24789 (2020-10-06)
A major challenge in converting lignocellulose to biofuel is overcoming the resistance of the biomass structure. Herein, sequential dilute acid-alkali/aqueous ammonia treatment was evaluated to enhance enzymatic hydrolysis of poplar biomass by removing hemicellulose first and then removing lignin with
Arun Sampathkumar et al.
Development (Cambridge, England), 146(10) (2019-05-12)
How organisms attain their specific shapes and modify their growth patterns in response to environmental and chemical signals has been the subject of many investigations. Plant cells are at high turgor pressure and are surrounded by a rigid yet flexible
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实验方案

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

纤维素酶的酶学测定

该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。

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