形式
liquid
质量水平
应用
BCIP/NBT 溶液，经过预混合后：
- 作为底物用于斑点印迹显影
- 作为底物用于在免疫组织化学分析中呈现碱性磷酸酶
- 在蛋白质印迹分析中检测特异性免疫复合物
其他说明
0.48mM NBT，0.56mM BCIP，10mM Tris 和 59.3mM MgCl2，pH 约 9.2
底物
用于在蛋白质印迹中显现碱性磷酸酶缀合物的底物。
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Willie Lin et al.
Oncotarget, 9(2), 1992-2001 (2018-02-09)
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PLoS ONE, 8(4), e61058-e61058 (2013)
The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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